Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPSA / Laminin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99714-100
Catalog Number: LS-G99714
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human 67kDa Laminin Receptor(RPSA) (NM_002295), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 37 kDa laminin receptor | 37LRP | 40S ribosomal protein SA | 67LR | 67 kDa laminin receptor | 37/67 kDa laminin receptor | 67kD | ribosomal protein SA | LAMR1 | LAMBR | Laminin receptor 1 | LamR | NEM/1CHD4 | RPSA | SA | LRP/LR | Laminin binding protein | Laminin Receptor | LRP | p40 | Ribosomal protein SA
- NCBI GenBank
- NM_002295
- SwissProt
- P08865
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL61AARAIVAIEN PADVSVISSR NTGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR121LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA IPCNNKGAHS VGLMWWMLAR181EVLRMRGTIS REHPWEVMPD LYFYRDPEEI EKEGQAAAEK AVTKEEFQGE WTAPAPEFTA241TQPEVADWSE GVQVPSVPIQ QFPTEDWSAQ PATEDWSAAP TAQATEWVGA TTDWSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
295 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.