Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RNF112 / ZNF179 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114818-100
Catalog Number: LS-G114818
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF179(RNF112) (NM_007148), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain finger protein | ZNF179 | Zinc finger protein 179 | Ring finger protein 112 | RNF112 | BFP
- Origin Species
- Human
- NCBI GenBank
- NM_007148
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRPALSVTS FCHRLGKRER KQSFMGNSGN SWSHTPFPKL ELGLGPQPMA PRELPTCSIC61LERLRDPISL DCGHDFCIRC FSTHRLPGCE PPCCPECRKI CKQKRGLRSL GEKMKLLPQR121PLPPALQETC PVRAEPLLLV RINASGGLIL RMGAINRCLK HPLARDTPVC LLAVLGEQHS181GKSFLLNHLL QGLPGLESGE GGRPRGGEAS LQGCRWGANG LARGIWMWSH PFLLGKEGKK241VAVFLVDTGD AMSPELSRET RIKLCALTTM LSSYQILSTS QELKDTDLDY LEMFVHVAEV301MGKHYGMVPI QHLDLLVRDS SHPNKAGQGH VGNIFQRLSG RYPKVQELLQ GKRARCCLLP361APGRRRMNQG HASPGDTDDD FRHLLGAYVS DVLSAAPQHA KSRCQGYWNE GRAVARGDRR421LLTGQQLAQE IKNLSGWMGR TGPGFTSPDE MAAQLHDLRK VEAAKREFEE YVRQQDVATK481RIFSALRVLP DTMRNLLSTQ KDAILARHGV ALLCKGRDQT LEALEAELQA TAKAFMDSYT541MRFCGHLAAV GGAVGAGLMG LAGGVVGAGM AAAALAAEAG MVAAGAAVGA TGAAVVGGGV601GAGLAATVGC MEKEEDERLL EGDREPLLQE ETRTRPLEQKLISEEDLAANDILDYKDDDDKV
631 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.