LSBio
Human RNF112 / ZNF179 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RNF112 / ZNF179 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RNF112 antibodies →
$153.00each
Catalog Number: LS-G91361-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF179(RNF112) (NM_007148) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Brain finger protein | ZNF179 | Zinc finger protein 179 | Ring finger protein 112 | RNF112 | BFP
Origin Species
Human
NCBI GenBank
NM_007148

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRPALSVTS FCHRLGKRER KQSFMGNSGN SWSHTPFPKL ELGLGPQPMA PRELPTCSIC
61LERLRDPISL DCGHDFCIRC FSTHRLPGCE PPCCPECRKI CKQKRGLRSL GEKMKLLPQR
121PLPPALQETC PVRAEPLLLV RINASGGLIL RMGAINRCLK HPLARDTPVC LLAVLGEQHS
181GKSFLLNHLL QGLPGLESGE GGRPRGGEAS LQGCRWGANG LARGIWMWSH PFLLGKEGKK
241VAVFLVDTGD AMSPELSRET RIKLCALTTM LSSYQILSTS QELKDTDLDY LEMFVHVAEV
301MGKHYGMVPI QHLDLLVRDS SHPNKAGQGH VGNIFQRLSG RYPKVQELLQ GKRARCCLLP
361APGRRRMNQG HASPGDTDDD FRHLLGAYVS DVLSAAPQHA KSRCQGYWNE GRAVARGDRR
421LLTGQQLAQE IKNLSGWMGR TGPGFTSPDE MAAQLHDLRK VEAAKREFEE YVRQQDVATK
481RIFSALRVLP DTMRNLLSTQ KDAILARHGV ALLCKGRDQT LEALEAELQA TAKAFMDSYT
541MRFCGHLAAV GGAVGAGLMG LAGGVVGAGM AAAALAAEAG MVAAGAAVGA TGAAVVGGGV
601GAGLAATVGC MEKEEDERLL EGDREPLLQE E
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

631 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.