Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RIP4 / ANKRD3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111097-100
Catalog Number: LS-G111097
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIPK4 (NM_020639), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANKK2 | Ankyrin repeat domain 3 | ANKRD3 | DIK | NKRD3 | RIP4 | PKK | PPS2 | RIPK4
- Origin Species
- Human
- NCBI GenBank
- NM_020639
- SwissProt
- P57078
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGDGGTPWA LALLRTFDAG EFTGWEKVGS GGFGQVYKVR HVHWKTWLAI KCSPSLHVDD61RERMELLEEA KKMEMAKFRY ILPVYGICRE PVGLVMEYME TGSLEKLLAS EPLPWDLRFR121IIHETAVGMN FLHCMAPPLL HLDLKPANIL LDAHYHVKIS DFGLAKCNGL SHSHDLSMDG181LFGTIAYLPP ERIREKSRLF DTKHDVYSFA IVIWGVLTQK KPFADEKNIL HIMVKVVKGH241RPELPPVCRA RPRACSHLIR LMQRCWQGDP RVRPTFQEIT SETEDLCEKP DDEVKETAHD301LDVKSPPEPR SEVVPARLKR ASAPTFDNDY SLSELLSQLD SGVSQAVEGP EELSRSSSES361KLPSSGSGKR LSGVSSVDSA FSSRGSLSLS FEREPSTSDL GTTDVQKKKL VDAIVSGDTS421KLMKILQPQD VDLALDSGAS LLHLAVEAGQ EECAKWLLLN NANPNLSNRR GSTPLHMAVE481RRVRGVVELL LARKISVNAK DEDQWTALHF AAQNGDESST RLLLEKNASV NEVDFEGRTP541MHVACQHGQE NIVRILLRRG VDVSLQGKDA WLPLHYAAWQ GHLPIVKLLA KQPGVSVNAQ601TLDGRTPLHL AAQRGHYRVA RILIDLCSDV NVCSLLAQTP LHVAAETGHT STARLLLHRG661AGKEAVTSDG YTALHLAARN GHLATVKLLV EEKADVLARG PLNQTALHLA AAHGHSEVVE721ELVSADVIDL FDEQGLSALH LAAQGRHAQT VETLLRHGAH INLQSLKFQG GHGPAATLLR781RSKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
784 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.