LSBio
Human RIP4 / ANKRD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RIP4 / ANKRD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RIPK4 antibodies →
$220.00each
Catalog Number: LS-G91437-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RIPK4 (NM_020639) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKK2 | Ankyrin repeat domain 3 | ANKRD3 | DIK | NKRD3 | RIP4 | PKK | PPS2 | RIPK4
Origin Species
Human
NCBI GenBank
NM_020639
SwissProt
P57078

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGDGGTPWA LALLRTFDAG EFTGWEKVGS GGFGQVYKVR HVHWKTWLAI KCSPSLHVDD
61RERMELLEEA KKMEMAKFRY ILPVYGICRE PVGLVMEYME TGSLEKLLAS EPLPWDLRFR
121IIHETAVGMN FLHCMAPPLL HLDLKPANIL LDAHYHVKIS DFGLAKCNGL SHSHDLSMDG
181LFGTIAYLPP ERIREKSRLF DTKHDVYSFA IVIWGVLTQK KPFADEKNIL HIMVKVVKGH
241RPELPPVCRA RPRACSHLIR LMQRCWQGDP RVRPTFQEIT SETEDLCEKP DDEVKETAHD
301LDVKSPPEPR SEVVPARLKR ASAPTFDNDY SLSELLSQLD SGVSQAVEGP EELSRSSSES
361KLPSSGSGKR LSGVSSVDSA FSSRGSLSLS FEREPSTSDL GTTDVQKKKL VDAIVSGDTS
421KLMKILQPQD VDLALDSGAS LLHLAVEAGQ EECAKWLLLN NANPNLSNRR GSTPLHMAVE
481RRVRGVVELL LARKISVNAK DEDQWTALHF AAQNGDESST RLLLEKNASV NEVDFEGRTP
541MHVACQHGQE NIVRILLRRG VDVSLQGKDA WLPLHYAAWQ GHLPIVKLLA KQPGVSVNAQ
601TLDGRTPLHL AAQRGHYRVA RILIDLCSDV NVCSLLAQTP LHVAAETGHT STARLLLHRG
661AGKEAVTSDG YTALHLAARN GHLATVKLLV EEKADVLARG PLNQTALHLA AAHGHSEVVE
721ELVSADVIDL FDEQGLSALH LAAQGRHAQT VETLLRHGAH INLQSLKFQG GHGPAATLLR
781RSKT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

784 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.