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RGS9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGS9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RGS9 antibodies →
$504.00each
Catalog Number: LS-G119768-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGS9 (NM_001081955), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PERRS | RGS9L | RGS9
Origin Species
Human
NCBI GenBank
NM_003835
SwissProt
O75916

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTGSDVLQWI VQRLWISSLE AQNLGNFIVR YGYIYPLQDP KNLILKPDGS LYRFQTPYFW
61PTQQWPAEDT DYAIYLAKRN IKKKGILEEY EKENYNFLNQ KMNYKWDFVI MQAKEQYRAG
121KERNKADRYA LDCQEKAYWL VHRCPPGMDN VLDYGLDRVT NPNEVKKQTV VAVKKEIMYY
181QQALMRSTVK SSVSLGGIVK YSEQFSSNDA IMSGCLPSNP WITDDTKFWD LNAKLVEIPT
241KMRVERWAFN FSELIRDPKG RQSFQYFLKK EFSGENLGFW EACEDLKYGD QSKVKEKAEE
301IYKLFLAPGA RRWINIDGKT MDITVKGLKH PHRYVLDAAQ THIYMLMKKD SYARYLKSPI
361YKDMLAKAIE PQETTKKSST LPFMRRHLRS SPSPVILRQL EEEAKAREAA NTVDITQPGQ
421HMAPSPHLTV YTGTCMPPSP SSPFSSSCRS PRKPFASPSR FIRRPSTTIC PSPIRVALES
481SSGLEQKGEC SGSMAPRGPS VTESSEASLD TSWPRSRPRA PPKARMALSF SRFLRRGCLA
541SPVFARLSPK CPAVSHGRVQ PLGDVGQQLP RLKSKRVANF FQIKMDVPTG SGTCLMDSED
601AGTGESGDRA TEKEVICPWE SL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

622 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.