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RGS9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGS9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RGS9 antibodies →
$725.00each
Catalog Number: LS-G117063-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGS9 (NM_003835), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PERRS | RGS9L | RGS9
Origin Species
Human
NCBI GenBank
NM_003835
SwissProt
O75916

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTIRHQGQQY RPRMAFLQKI EALVKDMQNP ETGVRMQNQR VLVTSVPHAM TGSDVLQWIV
61QRLWISSLEA QNLGNFIVRY GYIYPLQDPK NLILKPDGSL YRFQTPYFWP TQQWPAEDTD
121YAIYLAKRNI KKKGILEEYE KENYNFLNQK MNYKWDFVIM QAKEQYRAGK ERNKADRYAL
181DCQEKAYWLV HRCPPGMDNV LDYGLDRVTN PNEVKVNQKQ TVVAVKKEIM YYQQALMRST
241VKSSVSLGGI VKYSEQFSSN DAIMSGCLPS NPWITDDTQF WDLNAKLVEI PTKMRVERWA
301FNFSELIRDP KGRQSFQYFL KKEFSGENLG FWEACEDLKY GDQSKVKEKA EEIYKLFLAP
361GARRWINIDG KTMDITVKGL KHPHRYVLDA AQTHIYMLMK KDSYARYLKS PIYKDMLAKA
421IEPQETTKKS STLPFMRRHL RSSPSPVILR QLEEEAKARE AANTVDITQP GQHMAPSPHL
481TVYTGTCMPP SPSSPFSSSC RSPRKPFASP SRFIRRPSTT ICPSPIRVAL ESSSGLEQKG
541ECSGSMAPRG PSVTESSEAS LDTSWPRSRP RAPPKARMAL SFSRFLRRGC LASPVFARLS
601PKCPAVSHGR VQPLGDVGQQ LPRLKSKRVA NFFQIKMDVP TGSGTCLMDS EDAGTGESGD
661RATEKEVICP WESL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

674 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.