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RGS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112207-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGS3 (NM_017790), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2PA | RGP3 | PDZ-RGS3 | RGS3
Origin Species
Human
NCBI GenBank
NM_021106
SwissProt
P49796

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERSLHRVSL GSRRAHPDLS FYLTTFGQLR LSIDAQDRVL LLHIIEGKGL ISKQPGTCDP
61YVKISLIPED SRLRHQKTQT VPDCRDPAFH EHFFFPVQEE DDQKRLLVTV WNRASQSRQS
121GLIGCMSFGV KSLLTPDKEI SGWYYLLGEH LGRTKHLKVA RRRLRPLRDP LLRMPGGGDT
181ENGKKLKITI PRGKDGFGFT ICCDSPVRVQ AVDSGGPAER AGLQQLDTVL QLNERPVEHW
241KCVELAHEIR SCPSEIILLV WRMVPQVKPG PDGGVLRRAS CKSTHDLQSP PNKREKNCTH
301GVQARPEQRH SCHLVCDSSD GLLLGGWERY TEVAKRGGQH TLPALSRATA PTDPNYIILA
361PLNPGSQLLR PVYQEDTIPE ESGSPSKGKS YTGLGKKSRL MKTVQTMKGH GNYQNCPVVR
421PHATHSSYGT YVTLAPKVLV FPVFVQPLDL CNPARTLLLS EELLLYEGRN KAAEVTLFAY
481SDLLLFTKED EPGRCDVLRN PLYLQSVKLQ EGSSEDLKFC VLYLAEKAEC LFTLEAHSQE
541QKKRVCWCLS ENIAKQQQLA ASPPDSKKLH PFGSLQQEMG PVNSTNATQD RSFTSPGQTL
601IG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.