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RGS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101719-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGS3 (NM_021106), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2PA | RGP3 | PDZ-RGS3 | RGS3
Origin Species
Human
NCBI GenBank
NM_021106
SwissProt
P49796

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MFETEADEKR EMALEEGKGP GAEDSPPSKE PSPGQELPPG QDLPPNKDSP SGQEPAPSQE
61PLSSKDSATS EGSPPGPDAP PSKDVPPCQE PPPAQDLSPC QDLPAGQEPL PHQDPLLTKD
121LPAIQESPTR DLPPCQDLPP SQVSLPAKAL TEDTMSSGDL LAATGDPPAA PRPAFVIPEV
181RLDSTYSQKA GAEQGCSGDE EDAEEAEEVE EGEEGEEDED EDTSDDNYGE RSEAKRSSMI
241ETGQGAEGGL SLRVQNSLRR RTHSEGSLLQ EPRGPCFASD TTLHCSDGEG AASTWGMPSP
301STLKKELGRN GGSMHHLSLF FTGHRKMSGA DTVGDDDEAS RKRKSKNLAK DMKNKLGIFR
361RRNESPGAPP AGKADKMMKS FKPTSEEALK WGESLEKLLV HKYGLAVFQA FLRTEFSEEN
421LEFWLACEDF KKVKSQSKMA SKAKKIFAEY IAIQACKEVN LDSYTREHTK DNLQSVTRGC
481FDLAQKRIFG LMEKDSYPRF LRSDLYLDLI NQKKMSPPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

519 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.