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RGS12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGS12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RGS12 antibodies →
$725.00each
Catalog Number: LS-G103721-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGS12 (NM_198229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RGS12
Origin Species
Human
NCBI GenBank
NM_002926
SwissProt
O14924

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
156.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFRAGEASKR PLPGPSPPRV RSVEVARGRA GYGFTLSGQA PCVLSCVMRG SPADFVGLRA
61GDQILAVNEI NVKKASHEDV VKLIGKCSGV LHMVIAEGVG RFESCSSDEE GGLYEGKGWL
121KPKLDSKALG INRAERVVEE MQSGGIFNMI FENPSLCASN SEPLKLKQRS LSESAATRFD
181VGHESINNPN PNMLSKEEIS KVIHDDSVFS IGLESHDDFA LDASILNVAM IVGYLGSIEL
241PSTSSNLESD SLQAIRGCMR RLRAEQKIHS LVTMKIMHDC VQLSTDKAGV VAEYPAEKLA
301FSAVCPDDRR FFGLVTMQTN DDGSLAQEEE GALRTSCHVF MVDPDLFNHK IHQGIARRFG
361FECTADPDTN GCLEFPASSL PVLQFISVLY RDMGELIEGM RARAFLDGDA DAHQNNSTSS
421NSDSGIGNFH QEEKSNRVLV VDLGGSSSRH GPGGSAWDGV GGRGAQPWGA PWTGPFCPDP
481EGSPPFEAAH QTDRFWDLNK HLGPASPVEV PPASLRSSVP PSKRGTVGAG CGFNQRWLPV
541HVLREWQCGH TSDQDSYTDS TDGWSSINCG TLPPPMSKIP ADRYRVEGSF AQPPLNAPKR
601EWSRKAFGMQ SIFGPHRNVR KTKEDKKGSK FGRGTGLTQP SQRTSARRSF GRSKRFSITR
661SLDDLESATV SDGELTGADL KDCVSNNSLS SNASLPSVQS CRRLRERRVA SWAVSFERLL
721QDPVGVRYFS DFLRKEFSEE NILFWQACEY FNHVPAHDKK ELSYRAREIF SKFLCSKATT
781PVNIDSQAQL ADDVLRAPHP DMFKEQQLQI FNLMKFDSYT RFLKSPLYQE CILAEVEGRA
841LPDSQQVPSS PASKHSLGSD HSSVSTPKKL SGKSKSGRSL NEELGDEDSE KKRKGAFFSW
901SRTRSTGRSQ KKREHGDHAD DALHANGGLC RRESQGSVSS AGSLDLSEAC RTLAPEKDKA
961TKHCCIHLPD GTSCVVAVKA GFSIKDILSG LCERHGINGA AADLFLVGGD KPLVLHQDSS
1021ILESRDLRLE KRTLFRLDLV PINRSVGLKA KPTKPVTEVL RPVVARYGLD LSGLLVRLSG
1081EKEPLDLGAP ISSLDGQRVV LEEKDPSRGK ASADKQKGVP VKQNTAVNSS SRNHSATGEE
1141RTLGKSNSIK IKGENGKNAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN
1201RADDQRGLLR KEDLVLPEFL RLPPGSTELT LPTPAAVAKG FSKRSATGNG RESASQPGEQ
1261WEPVQESSDS PSTSPGSASS PPGPPGTTPP GQKSPSGPFC TPQSPVSLAQ EGTAQIWKRQ
1321SQEVEAGGIQ TVEDEHVAEL TLMGEGDISS PNSTLLPPPS TPQEVPGPSR PGSGTHGSRD
1381LPVNRIIDVD LVTGSAPGRD GGIAGAQAGP GRSQASGGPP TSDLPGLGPV PGEPAKPKTS
1441AHHATFV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1447 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.