RGS12 Antibody
Catalog Number: LS-C805328
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RGS12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89153-20
Catalog Number: LS-G89153
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RGS12 (NM_198229) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RGS12
- Origin Species
- Human
- NCBI GenBank
- NM_002926
- SwissProt
- O14924
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 156.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFRAGEASKR PLPGPSPPRV RSVEVARGRA GYGFTLSGQA PCVLSCVMRG SPADFVGLRA61GDQILAVNEI NVKKASHEDV VKLIGKCSGV LHMVIAEGVG RFESCSSDEE GGLYEGKGWL121KPKLDSKALG INRAERVVEE MQSGGIFNMI FENPSLCASN SEPLKLKQRS LSESAATRFD181VGHESINNPN PNMLSKEEIS KVIHDDSVFS IGLESHDDFA LDASILNVAM IVGYLGSIEL241PSTSSNLESD SLQAIRGCMR RLRAEQKIHS LVTMKIMHDC VQLSTDKAGV VAEYPAEKLA301FSAVCPDDRR FFGLVTMQTN DDGSLAQEEE GALRTSCHVF MVDPDLFNHK IHQGIARRFG361FECTADPDTN GCLEFPASSL PVLQFISVLY RDMGELIEGM RARAFLDGDA DAHQNNSTSS421NSDSGIGNFH QEEKSNRVLV VDLGGSSSRH GPGGSAWDGV GGRGAQPWGA PWTGPFCPDP481EGSPPFEAAH QTDRFWDLNK HLGPASPVEV PPASLRSSVP PSKRGTVGAG CGFNQRWLPV541HVLREWQCGH TSDQDSYTDS TDGWSSINCG TLPPPMSKIP ADRYRVEGSF AQPPLNAPKR601EWSRKAFGMQ SIFGPHRNVR KTKEDKKGSK FGRGTGLTQP SQRTSARRSF GRSKRFSITR661SLDDLESATV SDGELTGADL KDCVSNNSLS SNASLPSVQS CRRLRERRVA SWAVSFERLL721QDPVGVRYFS DFLRKEFSEE NILFWQACEY FNHVPAHDKK ELSYRAREIF SKFLCSKATT781PVNIDSQAQL ADDVLRAPHP DMFKEQQLQI FNLMKFDSYT RFLKSPLYQE CILAEVEGRA841LPDSQQVPSS PASKHSLGSD HSSVSTPKKL SGKSKSGRSL NEELGDEDSE KKRKGAFFSW901SRTRSTGRSQ KKREHGDHAD DALHANGGLC RRESQGSVSS AGSLDLSEAC RTLAPEKDKA961TKHCCIHLPD GTSCVVAVKA GFSIKDILSG LCERHGINGA AADLFLVGGD KPLVLHQDSS1021ILESRDLRLE KRTLFRLDLV PINRSVGLKA KPTKPVTEVL RPVVARYGLD LSGLLVRLSG1081EKEPLDLGAP ISSLDGQRVV LEEKDPSRGK ASADKQKGVP VKQNTAVNSS SRNHSATGEE1141RTLGKSNSIK IKGENGKNAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN1201RADDQRGLLR KEDLVLPEFL RLPPGSTELT LPTPAAVAKG FSKRSATGNG RESASQPGEQ1261WEPVQESSDS PSTSPGSASS PPGPPGTTPP GQKSPSGPFC TPQSPVSLAQ EGTAQIWKRQ1321SQEVEAGGIQ TVEDEHVAEL TLMGEGDISS PNSTLLPPPS TPQEVPGPSR PGSGTHGSRD1381LPVNRIIDVD LVTGSAPGRD GGIAGAQAGP GRSQASGGPP TSDLPGLGPV PGEPAKPKTS1441AHHATFVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1447 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
