Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RFTN1 / Raftlin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113414-100
Catalog Number: LS-G113414
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RFTN1 (NM_015150), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PIB10 | KIAA0084 | Raftlin | lipid raft linker 1 | RFTN1 | PIG9 | MIG2 | Raft-linking protein
- Origin Species
- Human
- NCBI GenBank
- D42043
- SwissProt
- Q14699
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGCGLNKLEK RDEKRPGNIY STLKRPQVET KIDVSYEYRF LEFTTLSAAE LPGSSAVRLA61SLRDLPAQLL ELYQQGFSLA ALHPFVQPTH EREKTPLEHI FRAILIKKTD RSQKTDLHNE121GYILELDCCS SLDHPTDQKL IPEFIKKIQE AASQGLKFVG VIPQYHSSVN SAGSSAPVST181ANSTEDARDA KNARGDHASL ENEKPGTGDV CSAPAGRNQS PEPSSGPRGE VPLAKQPSSP241SGEGDGGELS PQGVSKTLDG PESNPLEVHE EPLSGKMEIF TLFNKPKSHQ KCRQYYPVTI301PLHVSKNGQT VSGLDANWLE HMSDHFRKGG MLVNAVFYLG IVNDSLHGLT DGVFIFEAVS361TEDSKTIQGY DAIVVEQWTV LEGVEVQTDY VPLLNSLAAY GWQLTCVLPT PVVKTTSEGS421VSTKQIVFLQ RPCLPQKIKK KESKFQWRFS REEMHNRQMR KSKGKLSARD KQQAEENEKN481LEDQSSKAGD MGNCVSGQQQ EGGVSEEMKG PVQEDKGEQL SPGGLLCGVG VEGEAVQNGP541ASHSRALVGI CTGHSNPGED ARDGDAEEVR ELGTVEENSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
578 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.