LSBio
Human RFTN1 / Raftlin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RFTN1 / Raftlin Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RFTN1 antibodies →
$220.00each
Catalog Number: LS-G76657-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RFTN1 (NM_015150) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PIB10 | KIAA0084 | Raftlin | lipid raft linker 1 | RFTN1 | PIG9 | MIG2 | Raft-linking protein
Origin Species
Human
NCBI GenBank
D42043
SwissProt
Q14699

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCGLNKLEK RDEKRPGNIY STLKRPQVET KIDVSYEYRF LEFTTLSAAE LPGSSAVRLA
61SLRDLPAQLL ELYQQGFSLA ALHPFVQPTH EREKTPLEHI FRAILIKKTD RSQKTDLHNE
121GYILELDCCS SLDHPTDQKL IPEFIKKIQE AASQGLKFVG VIPQYHSSVN SAGSSAPVST
181ANSTEDARDA KNARGDHASL ENEKPGTGDV CSAPAGRNQS PEPSSGPRGE VPLAKQPSSP
241SGEGDGGELS PQGVSKTLDG PESNPLEVHE EPLSGKMEIF TLFNKPKSHQ KCRQYYPVTI
301PLHVSKNGQT VSGLDANWLE HMSDHFRKGG MLVNAVFYLG IVNDSLHGLT DGVFIFEAVS
361TEDSKTIQGY DAIVVEQWTV LEGVEVQTDY VPLLNSLAAY GWQLTCVLPT PVVKTTSEGS
421VSTKQIVFLQ RPCLPQKIKK KESKFQWRFS REEMHNRQMR KSKGKLSARD KQQAEENEKN
481LEDQSSKAGD MGNCVSGQQQ EGGVSEEMKG PVQEDKGEQL SPGGLLCGVG VEGEAVQNGP
541ASHSRALVGI CTGHSNPGED ARDGDAEEVR ELGTVEEN
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

578 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.