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RCOR3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RCOR3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RCOR3 antibodies →
$504.00each
Catalog Number: LS-G125703-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RCOR3(RCOR3) (NM_001136225), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1343 | REST corepressor 3 | RP11-318L16.1 | RCOR3
Origin Species
Human
SwissProt
Q9P2K3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MPGMMEKGPE LLGKNRSANG SAKSPAGGGG SGASSTNGGL HYSEPESGCS SDDEHDVGMR
61VGAEYQARIP EFDPGATKYT DKDNGGMLVW SPYHSIPDAK LDEYIAIAKE KHGYNVEQAL
121GMLFWHKHNI EKSLADLPNF TPFPDEWTVE DKVLFEQAFS FHGKSFHRIQ QMLPDKTIAS
181LVKYYYSWKK TRSRTSLMDR QARKLANRHN QGDSDDDVEE THPMDGNDSD YDPKKEAKKE
241GNTEQPVQTS KIGLGRREYQ SLQHRHHSQR SKCRPPKGMY LTQEDVVAVS CSPNAANTIL
301RQLDMELISL KRQVQNAKQV NSALKQKMEG GIEEFKPPES NQKINARWTT EEQLLAVQGV
361RKYGKDFQAI ADVIGNKTVG QVKNFFVNYR RRFNLEEVLQ EWEAEQGTQA SNGDASTLGE
421ETKSASNVPS GKSTDEEEEV CLCMEFELI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

449 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.