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RCOR3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RCOR3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111869-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RCOR3(RCOR3) (NM_018254), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1343 | REST corepressor 3 | RP11-318L16.1 | RCOR3
Origin Species
Human
SwissProt
Q9P2K3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MRVGAEYQAR IPEFDPGATK YTDKDNGGML VWSPYHSIPD AKLDEYIAIA KEKHGYNVEQ
61ALGMLFWHKH NIEKSLADLP NFTPFPDEWT VEDKVLFEQA FSFHGKSFHR IQQMLPDKTI
121ASLVKYYYSW KKTRSRTSLM DRQARKLANR HNQGDSDDDV EETHPMDGND SDYDPKKEAK
181KEGNTEQPVQ TSKIGLGRRE YQSLQHRHHS QRSKCRPPKG MYLTQEDVVA VSCSPNAANT
241ILRQLDMELI SLKRQVQNAK QVNSALKQKM EGGIEEFKPP ESNQKINARW TTEEQLLAVQ
301GVRKYGKDFQ AIADVIGNKT VGQVKNFFVN YRRRFNLEEV LQEWEAEQGT QASNGDASTL
361GEETKSASNV PSGKSTDEEE EAQTPQAPRT LGPSPPAPSS TPTPTAPIAT LNQPPPLLRP
421TLPAAPALHR QPPPLQQQAR FIQPRPTLNQ PPPPLIRPAN SMPPRLNPRP VLSTVGGQQP
481PSLIGIQTDS QSSLH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.