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RBM46 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM46 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106832-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM46 (NM_144979), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 68 | CT68 | RNA binding motif protein 46 | RNA-binding motif protein 46 | RBM46
Origin Species
Human
NCBI GenBank
NM_144979
SwissProt
Q8TBY0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNEENIDGTN GCSKVRTGIQ NEAALLALME KTGYNMVQEN GQRKFGGPPP GWEGPPPPRG
61CEVFVGKIPR DMYEDELVPV FERAGKIYEF RLMMEFSGEN RGYAFVMYTT KEEAQLAIRI
121LNNYEIRPGK FIGVCVSLDN CRLFIGAIPK EKKKEEILDE MKKVTEGVVD VIVYPSATDK
181TKNRGFAFVE YESHRAAAMA RRKLIPGTFQ LWGHTIQVDW ADPEKEVDEE TMQRVKVLYV
241RNLMISTTEE TIKAEFNKFK PGAVERVKKL RDYAFVHFFN REDAVAAMSV MNGKCIDGAS
301IEVTLAKPVN KENTWRQHLN GQISPNSENL IVFANKEESH PKTLGKLPTL PARLNGQHSP
361SPPEVERCTY PFYPGTKLTP ISMYSLKSNH FNSAVMHLDY YCNKNNWAPP EYYLYSTTSQ
421DGKVLLVYKI VIPAIANGSQ SYFMPDKLCT TLEDAKELAA QFTLLHLDYN FHRSSINSLS
481PVSATLSSGT PSVLPYTSRP YSYPGYPLSP TISLANGSHV GQRLCISNQA SFF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

533 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.