LSBio
Human RBM46 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RBM46 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RBM46 antibodies →
$153.00each
Catalog Number: LS-G85204-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RBM46 (NM_144979) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 68 | CT68 | RNA binding motif protein 46 | RNA-binding motif protein 46 | RBM46
Origin Species
Human
NCBI GenBank
NM_144979
SwissProt
Q8TBY0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNEENIDGTN GCSKVRTGIQ NEAALLALME KTGYNMVQEN GQRKFGGPPP GWEGPPPPRG
61CEVFVGKIPR DMYEDELVPV FERAGKIYEF RLMMEFSGEN RGYAFVMYTT KEEAQLAIRI
121LNNYEIRPGK FIGVCVSLDN CRLFIGAIPK EKKKEEILDE MKKVTEGVVD VIVYPSATDK
181TKNRGFAFVE YESHRAAAMA RRKLIPGTFQ LWGHTIQVDW ADPEKEVDEE TMQRVKVLYV
241RNLMISTTEE TIKAEFNKFK PGAVERVKKL RDYAFVHFFN REDAVAAMSV MNGKCIDGAS
301IEVTLAKPVN KENTWRQHLN GQISPNSENL IVFANKEESH PKTLGKLPTL PARLNGQHSP
361SPPEVERCTY PFYPGTKLTP ISMYSLKSNH FNSAVMHLDY YCNKNNWAPP EYYLYSTTSQ
421DGKVLLVYKI VIPAIANGSQ SYFMPDKLCT TLEDAKELAA QFTLLHLDYN FHRSSINSLS
481PVSATLSSGT PSVLPYTSRP YSYPGYPLSP TISLANGSHV GQRLCISNQA SFF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

533 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.