Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RBM26 / SE70 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110477-100
Catalog Number: LS-G110477
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM26 (NM_022118), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARRS2 | CTCL tumor antigen se70-2 | RBM26 | RP11-255E21.1 | RNA-binding motif protein 26 | RNA binding motif protein 26 | SE70-2 | ZC3H17 | C13orf10 | PRO1777 | RNA-binding protein 26
- Origin Species
- Human
- NCBI GenBank
- NM_022118
- SwissProt
- Q5T8P6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 110.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSKMIIENF EALKSWLSKT LEPICDADPS ALAKYVLALV KKDKSEKELK ALCIDQLDVF61LQKETQIFVE KLFDAVNTKS YLPPPEQPSS GSLKVEFFPH QEKDIKKEEI TKEEEREKKF121SRRLNHSPPQ SSSRYRENRS RDERKKDDRS RKRDYDRNPP RRDSYRDRYN RRRGRSRSYS181RSRSRSWSKE RLRERDRDRS RTRSRSRTRS RERDLVKPKY DLDRTDPLEN NYTPVSSVPS241ISSGHYPVPT LSSTITVIAP THHGNNTTES WSEFHEDQVD HNSYVRPPMP KKRCRDYDEK301GFCMRGDMCP FDHGSDPVVV EDVNLPGMLP FPAQPPVVEG PPPPGLPPPP PILTPPPVNL361RPPVPPPGPL PPSLPPVTGP PPPLPPLQPS GMDAPPNSAT SSVPTVVTTG IHHQPPPAPP421SLFTADTYDT DGYNPEAPSI TNTSRPMYRH RVHAQRPNLI GLTSGDMDLP PREKPPNKSS481MRIVVDSESR KRTIGSGEPG VPTKKTWFDK PNFNRTNSPG FQKKVQFGNE NTKLELRKVP541PELNNISKLN EHFSRFGTLV NLQVAYNGDP EGALIQFATY EEAKKAISST EAVLNNRFIK601VYWHREGSTQ QLQTTSPKPL VQQPILPVVK QSVKERLGPV PSSTIEPAEA QSASSDLPQV661LSTSTGLTKT VYNPAALKAA QKTLLVSTSA VDNNEAQKKK QEALKLQQDV RKRKQEILEK721HIETQKMLIS KLEKNKTMKS EDKAEIMKTL EVLTKNITKL KDEVKAASPG RCLPKSIKTK781TQMQKELLDT ELDLYKKMQA GEEVTELRRK YTELQLEAAK RGILSSGRGR GIHSRGRGAV841HGRGRGRGRG RGVPGHAVVD HRPRALEISA FTESDREDLL PHFAQYGEIE DCQIDDSSLH901AVITFKTRAE AEAAAVHGAR FKGQDLKLAW NKPVTNISAV ETEEVEPDEE EFQEESLVDD961SLLQDDDEEE EDNESRSWRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
980 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.