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RBM10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBM10 antibodies →
$504.00each
Catalog Number: LS-G115808-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM10 (NM_005676), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GPATC9 | KIAA0122 | RNA-binding motif protein 10 | ZRANB5 | RNA binding motif protein 10 | RNA-binding protein 10 | RNA-binding protein S1-1 | TARPS | DXS8237E | GPATCH9 | RBM10 | S1-1
Origin Species
Human
NCBI GenBank
NM_005676
SwissProt
P98175

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEYERRGGRG DRTGRYGATD RSQDDGGENR SRDHDYRDMD YRSYPREYGS QEGKHDYDDS
61SEEQSAEDSY EASPGSETQR RRRRRHRHSP TGPPGFPRDG DYRDQDYRTE QGEEEEEEED
121EEEEEKASNI VMLRMLPQAA TEDDIRGQLQ SHGVQAREVR LMRNKSSGQS RGFAFVEFSH
181LQDATRWMEA NQHSLNILGQ KVSMHYSDPK PKINEDWLCN KCGVQNFKRR EKCFKCGVPK
241SEAEQKLPLG TRLDQQTLPL GGRELSQGLL PLPQPYQAQG VLASQALSQG SEPSSENAND
301TIILRNLNPH STMDSILGAL APYAVLSSSN VRVIKDKQTQ LNRGFAFIQL STIVEAAQLL
361QILQALHPPL TIDGKTINVE FAKGSKRDMA SNEGSRISAA SVASTAIAAA QWAISQASQG
421GEGTWATSEE PPVDYSYYQQ DEGYGNSQGT ESSLYAHGYL KGTKGPGITG TKGDPTGAGP
481EASLEPGADS VSMQAFSRAQ PGAAPGIYQQ SAEASSSQGT AANSQSYTIM SPAVLKSELQ
541SPTHPSSALP PATSPTAQES YSQYPVPDVS TYQYDETSGY YYDPQTGLYY DPNSQYYYNA
601QSQQYLYWDG ERRTYVPALE QSADGHKETG APSKEGKEKK EKHKTKTAQQ IAKDMERWAR
661SLNKQKENFK NSFQPISSLR DDERRESATA DAGYAILEKK GALAERQHTS MDLPKLASDD
721RPSPPRGLVA AYSGESDSEE EQERGGPERE EKLTDWQKLA CLLCRRQFPS KEALIRHQQL
781SGLHKQNLEI HRRAHLSENE LEALEKNDME QMKYRDRAAE RREKYGIPEP PEPKRRKYGG
841ISTASVDFEQ PTRDGLGSDN IGSRMLQAMG WKEGSGLGRK KQGIVTPIEA QTRVRGSGLG
901ARGSSYGVTS TESYKETLHK TMVTRFNEAQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

930 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.