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RBM10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBM10 antibodies →
$504.00each
Catalog Number: LS-G105913-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM10 (NM_152856), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GPATC9 | KIAA0122 | RNA-binding motif protein 10 | ZRANB5 | RNA binding motif protein 10 | RNA-binding protein 10 | RNA-binding protein S1-1 | TARPS | DXS8237E | GPATCH9 | RBM10 | S1-1
Origin Species
Human
NCBI GenBank
NM_005676
SwissProt
P98175

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEYERRGGRG DRTGRYGATD RSQDDGGENR SRDHDYRDMD YRSYPREYGS QEGKHDYDDS
61SEEQSAEIRG QLQSHGVQAR EVRLMRNKSS GQSRGFAFVE FSHLQDATRW MEANQHSLNI
121LGQKVSMHYS DPKPKINEDW LCNKCGVQNF KRREKCFKCG VPKSEAEQKL PLGTRLDQQT
181LPLGGRELSQ GLLPLPQPYQ AQGVLASQAL SQGSEPSSEN ANDTIILRNL NPHSTMDSIL
241GALAPYAVLS SSNVRVIKDK QTQLNRGFAF IQLSTIEAAQ LLQILQALHP PLTIDGKTIN
301VEFAKGSKRD MASNEGSRIS AASVASTAIA AAQWAISQAS QGGEGTWATS EEPPVDYSYY
361QQDEGYGNSQ GTESSLYAHG YLKGTKGPGI TGTKGDPTGA GPEASLEPGA DSVSMQAFSR
421AQPGAAPGIY QQSAEASSSQ GTAANSQSYT IMSPAVLKSE LQSPTHPSSA LPPATSPTAQ
481ESYSQYPVPD VSTYQYDETS GYYYDPQTGL YYDPNSQYYY NAQSQQYLYW DGERRTYVPA
541LEQSADGHKE TGAPSKEGKE KKEKHKTKTA QQIAKDMERW ARSLNKQKEN FKNSFQPISS
601LRDDERRESA TADAGYAILE KKGALAERQH TSMDLPKLAS DDRPSPPRGL VAAYSGESDS
661EEEQERGGPE REEKLTDWQK LACLLCRRQF PSKEALIRHQ QLSGLHKQNL EIHRRAHLSE
721NELEALEKND MEQMKYRDRA AERREKYGIP EPPEPKRRKY GGISTASVDF EQPTRDGLGS
781DNIGSRMLQA MGWKEGSGLG RKKQGIVTPI EAQTRVRGSG LGARGSSYGV TSTESYKETL
841HKTMVTRFNE AQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

852 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.