Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RASGRF1 / CDC25 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117691-100
Catalog Number: LS-G117691
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASGRF1 (NM_002891), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC25 | CDC25L | GNRP | GRF55 | H-GRF55 | PP13187 | RASGRF1 | CDC25 homolog | GRF1 | Ras-GRF1
- Origin Species
- Human
- NCBI GenBank
- NM_002891
- SwissProt
- Q13972
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 145.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL LFYFESDSSS61RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS HENQKALELR TEDAKDCDEW121VAAIAHASYR TLATEHEALM QKYLHLLQIV ETEKTVAKQL RQQIEDGEIE IERLKAEITS181LLKDNERIQS TQTVAPNDED SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK241RNQVVFSMLE AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH301QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC KQNRDFDKLL361KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP HEHVERNSLD YAKSKLEELS421RIMHDEVSET ENIRKNLAIE RMIIEGCEIL LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG481SLSLKKEGER QCFLFSKHLI ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG541QDIDHLDFKI GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS601KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY ASVERLLERL661TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS AIPARWLRSL ELLFASGQNN721KLLYGEPPKS PRATRKFSSP PPLSITKTSS PSRRRKLSLN IPIITGGKAL DLAALSCNSN781GYTSMYSAMS PFSKATLDTS KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE841ESDIDQNQSD DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL901SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA TNRVLNVLRH961WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA ANIIRTLTQE DPGDNQITLE1021EITQMAEGVK AEPFENHSAL EIAEQLTLLD HLVFKKIPYE EFFGQGWMKL EKNERTPYIM1081KTTKHFNDIS NLIASEIIRN EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA1141IFRLKKTWLK VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI1201EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD QSFVMDEESL1261YESSLRIEPK LPTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1273 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.