Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RASGRF1 / CDC25 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105693-100
Catalog Number: LS-G105693
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASGRF1 (NM_153815), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC25 | CDC25L | GNRP | GRF55 | H-GRF55 | PP13187 | RASGRF1 | CDC25 homolog | GRF1 | Ras-GRF1
- Origin Species
- Human
- NCBI GenBank
- NM_002891
- SwissProt
- Q13972
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MYSAMSPFSK ATLDTSKLYV SSSFTNKIPD EGDTTPEKPE DPSALSKQSS EVSMREESDI61DQNQSDDGDT ETSPTKSPTT PKSVKNKNSS EFPLFSYNNG VVMTSCRELD NNRSALSAAS121AFAIATAGAN EGTPNKEKYR RMSLASAGFP PDQRNGDKEF VIRRAATNRV LNVLRHWVSK181HSQDFETNDE LKCKVIGFLE EVMHDPELLT QERKAAANII RTLTQEDPGD NQITLEEITQ241MAEGVKAEPF ENHSALEIAE QLTLLDHLVF KKIPYEEFFG QGWMKLEKNE RTPYIMKTTK301HFNDISNLIA SEIIRNEDIN ARVSAIEKWV AVADICRCLH NYNAVLEITS SMNRSAIFRL361KKTWLKVSKQ TKALIDKLQK LVSSEGRFKN LREALKNCDP PCVPYLGMYL TDLAFIEEGT421PNYTEDGLVN FSKMRMISHI IREIRQFQQT AYKIEHQAKV TQYLLDQSFV MDEESLYESS481LRIEPKLPTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
489 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.