Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAPGEF5 / GFR Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114521-100
Catalog Number: LS-G114521
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAPGEF5 (NM_012294), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GFR | KIAA0277 | M-Ras-regulated Rap GEF | M-Ras-regulated GEF | MRGEF | RAPGEF5 | Related to Epac | REPAC | MR-GEF
- Origin Species
- Human
- NCBI GenBank
- D87467
- SwissProt
- Q92565
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAIGVWQLLL DMGIMLSVDQ HLYFQDTYVF YQFSSDECSY LYCEFEREEE WQNGVKLLLQ61LVPLIPARGG ICELSHQKIE DSEESSDEIL VRLTSAVQRE LAAVIALKAR KSAIEQDEEN121NDKHVAVTEA ESVPDSQAGV MCKLQERDEI GRIELVQKLA KENYQFLQTD KKEQEKSEHQ181DDEVTTVQVK EQDQSVLVLK KVQCCGPAPT AGSAESHWRY VVVSGTPEKI LEHLLNDLHL241EEVQDKETET LLDDFLLTYT VFMTTDDLCQ ALLRHYSAKK YQGKEENSDV PRRKRKVLHL301VSQWIALYKD WLPEDEHSKM FLKTIYRNVL DDVYEYPILE KELKEFQKIL GMHRRHTVDE361YSPQKKNKAL FHQFSLKENW LQHRGTVTET EEIFCHVYIT EHSYVSVKAK VSSIAQEILK421VVAEKIQYAE EDLALVAITF SGEKHELQPN DLVISKSLEA SGRIYVYRKD LADTLNPFAE481NEESQQRSMR ILGMNTWDLA LELMNFDWSL FNSIHEQELI YFTFSRQGSG EHTANLSLLL541QRCNEVQLWV ATEILLCSQL GKRVQLVKKF IKIAAHCKAQ RNLNSFFAIV MGLNTASVSR601LSQTWEKIPG KFKKLFSELE SLTDPSLNHK AYRDAFKKMK PPKIPFMPLL LKDVTFIHEG661NKTFLDNLVN FEKLHMIADT VRTLRHCRTN QFGDLSPKEH QELKSYVNHL YVIDSQQALF721ELSHRIEPRVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
730 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.