RAPGEF5 / GFR Antibody
Catalog Number: LS-C824474-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RAPGEF5 / GFR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G81059-20
Catalog Number: LS-G81059
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RAPGEF5 (NM_012294) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GFR | KIAA0277 | M-Ras-regulated Rap GEF | M-Ras-regulated GEF | MRGEF | RAPGEF5 | Related to Epac | REPAC | MR-GEF
- Origin Species
- Human
- NCBI GenBank
- D87467
- SwissProt
- Q92565
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAIGVWQLLL DMGIMLSVDQ HLYFQDTYVF YQFSSDECSY LYCEFEREEE WQNGVKLLLQ61LVPLIPARGG ICELSHQKIE DSEESSDEIL VRLTSAVQRE LAAVIALKAR KSAIEQDEEN121NDKHVAVTEA ESVPDSQAGV MCKLQERDEI GRIELVQKLA KENYQFLQTD KKEQEKSEHQ181DDEVTTVQVK EQDQSVLVLK KVQCCGPAPT AGSAESHWRY VVVSGTPEKI LEHLLNDLHL241EEVQDKETET LLDDFLLTYT VFMTTDDLCQ ALLRHYSAKK YQGKEENSDV PRRKRKVLHL301VSQWIALYKD WLPEDEHSKM FLKTIYRNVL DDVYEYPILE KELKEFQKIL GMHRRHTVDE361YSPQKKNKAL FHQFSLKENW LQHRGTVTET EEIFCHVYIT EHSYVSVKAK VSSIAQEILK421VVAEKIQYAE EDLALVAITF SGEKHELQPN DLVISKSLEA SGRIYVYRKD LADTLNPFAE481NEESQQRSMR ILGMNTWDLA LELMNFDWSL FNSIHEQELI YFTFSRQGSG EHTANLSLLL541QRCNEVQLWV ATEILLCSQL GKRVQLVKKF IKIAAHCKAQ RNLNSFFAIV MGLNTASVSR601LSQTWEKIPG KFKKLFSELE SLTDPSLNHK AYRDAFKKMK PPKIPFMPLL LKDVTFIHEG661NKTFLDNLVN FEKLHMIADT VRTLRHCRTN QFGDLSPKEH QELKSYVNHL YVIDSQQALF721ELSHRIEPRVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
730 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
