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RAPGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RAPGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103570-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRF2(RAPGEF1) (NM_198679), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C3G | CRK SH3-binding GNRP | GRF2 | Protein C3G | RAPGEF1
Origin Species
Human
NCBI GenBank
NM_005312
SwissProt
Q13905

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
122.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNAIEKQKP LKRSHLYPWK QDSQRSHLSS FTMKLMDKFH SPKIKRTPSK KGKPAEVSVK
61IPEKPVNKEA TDRFLPEGYP LPLDLEQQAV EFMSTSAVAS RSQRQKNLSW LEEKEKEVVS
121ALRYFKTIVD KMAIDKKVLE MLPGSASKVL EAILPLVQND PRIQHSSALS SCYSRVYQSL
181ANLIRWSDQV MLEGVNSEDK EMVTTVKGVI KAVLDGVKEL VRLTIEKQGR PSPTSPVKPS
241SPASKPDGPA ELPLTDREVE ILNKTTGMSQ STELLPDATD EEVAPPKPPL PGIRVVDNSP
301PPALPPKKRQ SAPSPTRVAV VAPMSRATSG SSLPVGINRQ DFDVDCYAQR RLSGGSHSYG
361GESPRLSPCS SIGKLSKSDE QLSSLDRDSG QCSRNTSCET LDHYDPDYEF LQQDLSNADQ
421IPQQTAWNLS PLPESLGESG SPFLGPPFQL PLGGHPQPDG PLAPGQQTDT PPALPEKKRR
481SAASQTADGS GCRVSYERHP SQYDNISGED LQSTAPIPSV PYAPFAAILP FQHGGSSAPV
541EFVGDFTAPE STGDPEKPPP LPEKKNKHML AYMQLLEDYS EPQPSMFYQT PQNEHIYQQK
601NKLLMEVYGF SDSFSGVDSV QELAPPPALP PKQRQLEPPA GKDGHPRDPS AVSGVPGKDS
661RDGSERAPKS PDALESAQSE EEVDELSLID HNEIMSRLTL KQEGDDGPDV RGGSGDILLV
721HATETDRKDL VLYCEAFLTT YRTFISPEEL IKKLQYRYEK FSPFADTFKK RVSKNTFFVL
781VRVVDELCLV ELTEEILKLL MELVFRLVCN GELSLARVLR KNILDKVDQK KLLRCATSSQ
841PLAARGVAAR PGTLHDFHSH EIAEQLTLLD AELFYKIEIP EVLLWAKEQN EEKSPNLTQF
901TEHFNNMSYW VRSIIMLQEK AQDRERLLLK FIKIMKHLRK LNNFNSYLAI LSALDSAPIR
961RLEWQKQTSE GLAEYCTLID SSSSFRAYRA ALSEVEPPCI PYLGLILQDL TFVHLGNPDY
1021IDGKVNFSKR WQQFNILDSM RCFQQAHYDM RRNDDIINFF NDFSDHLAEE ALWELSLKIK
1081PRNITRRKTD REEKT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1095 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.