RAPGEF1 Antibody
Catalog Number: LS-C823007-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RAPGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80815-20
Catalog Number: LS-G80815
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GRF2(RAPGEF1) (NM_198679) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C3G | CRK SH3-binding GNRP | GRF2 | Protein C3G | RAPGEF1
- Origin Species
- Human
- NCBI GenBank
- NM_005312
- SwissProt
- Q13905
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 122.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNAIEKQKP LKRSHLYPWK QDSQRSHLSS FTMKLMDKFH SPKIKRTPSK KGKPAEVSVK61IPEKPVNKEA TDRFLPEGYP LPLDLEQQAV EFMSTSAVAS RSQRQKNLSW LEEKEKEVVS121ALRYFKTIVD KMAIDKKVLE MLPGSASKVL EAILPLVQND PRIQHSSALS SCYSRVYQSL181ANLIRWSDQV MLEGVNSEDK EMVTTVKGVI KAVLDGVKEL VRLTIEKQGR PSPTSPVKPS241SPASKPDGPA ELPLTDREVE ILNKTTGMSQ STELLPDATD EEVAPPKPPL PGIRVVDNSP301PPALPPKKRQ SAPSPTRVAV VAPMSRATSG SSLPVGINRQ DFDVDCYAQR RLSGGSHSYG361GESPRLSPCS SIGKLSKSDE QLSSLDRDSG QCSRNTSCET LDHYDPDYEF LQQDLSNADQ421IPQQTAWNLS PLPESLGESG SPFLGPPFQL PLGGHPQPDG PLAPGQQTDT PPALPEKKRR481SAASQTADGS GCRVSYERHP SQYDNISGED LQSTAPIPSV PYAPFAAILP FQHGGSSAPV541EFVGDFTAPE STGDPEKPPP LPEKKNKHML AYMQLLEDYS EPQPSMFYQT PQNEHIYQQK601NKLLMEVYGF SDSFSGVDSV QELAPPPALP PKQRQLEPPA GKDGHPRDPS AVSGVPGKDS661RDGSERAPKS PDALESAQSE EEVDELSLID HNEIMSRLTL KQEGDDGPDV RGGSGDILLV721HATETDRKDL VLYCEAFLTT YRTFISPEEL IKKLQYRYEK FSPFADTFKK RVSKNTFFVL781VRVVDELCLV ELTEEILKLL MELVFRLVCN GELSLARVLR KNILDKVDQK KLLRCATSSQ841PLAARGVAAR PGTLHDFHSH EIAEQLTLLD AELFYKIEIP EVLLWAKEQN EEKSPNLTQF901TEHFNNMSYW VRSIIMLQEK AQDRERLLLK FIKIMKHLRK LNNFNSYLAI LSALDSAPIR961RLEWQKQTSE GLAEYCTLID SSSSFRAYRA ALSEVEPPCI PYLGLILQDL TFVHLGNPDY1021IDGKVNFSKR WQQFNILDSM RCFQQAHYDM RRNDDIINFF NDFSDHLAEE ALWELSLKIK1081PRNITRRKTD REEKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1095 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
