Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAP1GDS1 / SmgGDS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118915-100
Catalog Number: LS-G118915
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAP1GDS1 (NM_001100429), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Exchange factor smgGDS | GDS1 | RAP1GDS1 | SMG GDS protein | SmgGDS
- Origin Species
- Human
- NCBI GenBank
- NM_021159
- SwissProt
- P52306
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDNLSDTLKK LKITAVDKTE DSLEGCLDCL LQALAQNNTE TSEKIQASGI LQLFASLLTP61QSSCKAKVAN IIAEVAKNEF MRIPCVDAGL ISPLVQLLNS KDQEVLLQTG RALGNICYDS121HSLQAQLINM GVIPTLVKLL GIHCQNAALT EMCLVAFGNL AELESSKEQF ASTNIAEELV181KLFKKQIEHD KREMIFEVLA PLAENDAIKL QLVEAGLVEC LLEIVQQKVD SDKEDDITEL241KTGSDLMVLL LLGDESMQKL FEGGKGSVFQ RVLSWIPSNN HQLQLAGALA IANFARNDAN301CIHMVDNGIV EKLMDLLDRH VEDGNVTVQH AALSALRNLA IPVINKAKML SAGVTEAVLK361FLKSEMPPVQ FKLLGTLRML IDAQAEAAEQ LGKNVKLVER LVEWCEAKDH AGVMGESNRL421LSALIRHSKS KDVIKTIVQS GGIKHLVTMA TSEHVIMQNE ALVALALIAA LELGTAEKDL481ESAKLVQILH RLLADERSAP EIKYNSMVLI CALMGSECLH KEVQDLAFLD VVSKLRSHEN541KSVAQQASLT EQRLTVESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
558 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.