Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAP1GDS1 / SmgGDS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110737-100
Catalog Number: LS-G110737
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAP1GDS1 (NM_021159), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Exchange factor smgGDS | GDS1 | RAP1GDS1 | SMG GDS protein | SmgGDS
- Origin Species
- Human
- NCBI GenBank
- NM_021159
- SwissProt
- P52306
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADNLSDTLK KLKITAVDKT EDSLEGCLDC LLQALAQNNT ETSEKIQASG ILQLFATLLT61PQSSCKAKVA NIIAEVAKNE FMRIPCVDAG LISPLVQLLN SKDQEVLLQT GRALGNICYD121SHEGRSAVDQ AGGAQIVIDH LRSLCSITDP ANEKLLTVFC GMLMNYSNEN DSLQAQLINM181GVIPTLVKLL GIHCQNAALT EMCLVAFGNL AELESSKEQF ASTNIAEELV KLFKKQIEHD241KREMIFEVLA PLAENDAIKL QLVEAGLVEC LLEIVQQKVD SDKEDDITEL KTGSDLMVLL301LLGDESMQKL FEGGKGSVFQ RVLSWIPSNN HQLQLAGALA IANFARNDAN CIHMVDNGIV361EKLMDLLDRH VEDGNVTVQH AALSALRNLA IPVINKAKML SAGVTEAVLK FLKSEMPPVQ421FKLLGTLRML IDAQEAAEQL GKNVKLVERL VEWCEAKDHA GVMGESNRLL SALIRHSKSK481DVIKTIVQSG GIKHLVTMAT SEHVIMQNEA LVALALIAAL ELGTAEKDLE SAKLVQILHR541LLADERSAPE IKYNSMVLIC ALMGSECLHK EVQDLAFLDV VSKLRSHENK SVAQQASLTE601QRLTVESTRT RPLEQKLISE EDLAANDILD YKDDDDKV
638 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.