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PWWP2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PWWP2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119287-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PWWP domain containing protein 2B(PWWP2B) (NM_001098637), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA432J24.1 | RP11-273H7.1 | PWWP2B | Pp8607 | PWWP domain containing 2 | PWWP domain containing 2B | PWWP2
Origin Species
Human
NCBI GenBank
BF568430
SwissProt
Q6NUJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPRAGCRLP VRVEQVVNGA LVVTVSCGER SFAGILLDCT KKSGLFGLPP LAPLPQVDES
61PVNDSHGRAP EEGDAEVMQL GSSSPPPARG VQPPETTRPE PPPPLVPPLP AGSLPPYPPY
121FEGAPFPHPL WLRDTYKLWV PQPPPRTIKR TRRRLSRNRD PGRLILSTIR LRPRQVLCEK
181CKSTLSPPEA SPGPPAAPRA RRRLGSGPDR ELRKPEEPEN GEPTAAATAR RSKRERREED
241RAPAEQVPRS PVIKISYSTP QGKGEVVKIP SRVHGSLEPF RPQQAPQDDG SQDPEVLDRE
301SRDRPSCAPS ASIPKLKLTR PVPAGADLPP PKIRLKPHRL GDSEHEPVYR AELVGELNGY
361LRDSSPAPCA DGPAGGLADL SSGSSGEDDD FKSCPQGPQG REGLAFLVSC PEGRADCASE
421SACSSDSLDE ARSSGSEGTP ADTGDLSPGH GASAPSVSRE ARQTVPPLTV RLHTQSVSEC
481ITEDGRTVAV GDIVWGHRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

499 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.