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PWWP2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PWWP2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107297-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PWWP domain containing protein 2B(PWWP2B) (NM_138499), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA432J24.1 | RP11-273H7.1 | PWWP2B | Pp8607 | PWWP domain containing 2 | PWWP domain containing 2B | PWWP2
Origin Species
Human
NCBI GenBank
BF568430
SwissProt
Q6NUJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQLGSSSPPP ACGVQPPETT GPEPPPPLVP PLPAGSLPPY PPYFEGAPFP HPLWLRDTYK
61LWVPQPPPRT IKRTRRRLSR NRDPGRLILS TIRLRPRQVL CEKCKSTLSP PEASPGPPAA
121PRARRRLGSG PDRELRKPEE PENGEPTAAA TARRSKRERR EEDRAPAEQV PRSPVIKISY
181STPQGKGEVV KIPSRVHGSL EPFRPQQAPQ DDGSQDPEVL DRESRDRPSC APSASIPKLK
241LTRPVPAGAD LPPPKIRLKP HRLGDSEHEP VYRAELVGEL NGYLRDSSPA PCADGPAGGL
301ADLSSGSSGE DDDFKSCPQG PQGREGLAFL VSCPEGRADC ASESACSSDS LDEARSSGSE
361GTPADTGDLS PGHGASAPSV SREARQTVPP LTVRLHTQSV SECITEDGRT VAVGDIVWGK
421IHGFPWWPAR VLDISLGQKE DGEPSWREAK VSWFGSPTTS FLSISKLSPF SEFFKLRFNR
481KKKGMYRKAI TEAANAARHV APEIRELLTQ FET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.