Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PV1 / PLVAP Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109253-100
Catalog Number: LS-G109253
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLVAP (NM_031310), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- gp68 | FELS | Plasmalemma vesicle protein 1 | PV-1 | PLVAP | PV1
- Origin Species
- Human
- NCBI GenBank
- NM_031310
- SwissProt
- Q9BX97
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLAMEHGGS YARAGGSSRG CWYYLRYFFL FVSLIQFLII LGLVLFMVYG NVHVSTESNL61QATERRAEGL YSQLLGLTAS QSNLTKELNF TTRAKDAIMQ MWLNARRDLD RINASFRQCQ121GDRVIYTNNQ RYMAAIILSE KQCRDQFKDM NKSCDALLFM LNQKVKTLEV EIAKEKTICT181KDKESVLLNK RVAEEQLVEC VKTRELQHQE RQLAKEQLQK VQALCLPLDK DKFEMDLRNL241WRDSIIPRSL DNLGYNLYHP LGSELASIRR ACDHMPSLMS SKVEELARSL RADIERVARE301NSDLQRQKLE AQQGLRASQE AKQKVEKEAQ AREAKLQAEC SRQTQLALEE KAVLRKERDN361LAKELEEKKR EAEQLRMELA IRNSALDTCI KTKSQPMMPV SRPMGPVPNP QPIDPASLEE421FKRKILESQR PPAGIPVAPS SGSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
442 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.