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PUM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PUM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113318-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Pumilio 2(PUM2) (NM_015317), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0235 | PUM2 | Pumilio homolog 2 (Drosophila) | PUMH2 | Pumilio (Drosphila) homolog 2 | Pumilio-2 | Pumilio homolog 2 | PUML2
Origin Species
Human
NCBI GenBank
NM_015317
SwissProt
Q8TB72

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW GASHHSMSQP
61IMVQRRSGQG FHGNSEVNAI LSPRSESGGL GVSMVEYVLS SSPADKLDSR FRKGNFGTRD
121AETDGPEKGD QKGKASPFEE DQNRDLKQGD DDDSKINGRG LPNGMDADCK DFNRTPGSRQ
181ASPTEVVERL GPNTNPSEGL GPLPNPTANK PLVEEFSNPE TQNLDAMEQV GLESLQFDYP
241GNQVPMDSSG ATVGLFDYNS QQQLFQRTNA LTVQQLTAAQ QQQYALAAAQ QPHIAGVFSA
301GLAPAAFVPN PYIISAAPPG TDPYTAAGLA AAATLAGPAV VPPQYYGVPW GVYPANLFQQ
361QAAAAANNTA SQQAASQAQP GQQQVLRAGA GQRPLTPNQG QQGQQAESLA AAAAANPTLA
421FGQGLATGMP GYQVLAPTAY YDQTGALVVG PGARTGLGAP VRLMAPTPVL ISSAAAQAAA
481AAAAGGTASS LTGSTNGLFR PIGTQPPQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS
541HGPGQPGSTS LGFGSGNSLG AAIGSALSGF GSSVGSSASS SATRRESLST SSDLYKRSSS
601SLAPIGQPFY NSLGFSSSPS PIGMPLPSQT PGHSLTPPPS LSSHGSSSSL HLGGLTNGSG
661RYISAAPGAE AKYRSASSTS SLFSSSSQLF PPSRLRYNRS DIMPSGRSRL LEDFRNNRFP
721NLQLRDLIGH IVEFSQDQHG SRFIQQKLER ATPAERQMVF NEILQAAYQL MTDVFGNYVI
781QKFFEFGSLD QKLALATRIR GHVLPLALQM YGCRVIQKAL ESISSDQQSE MVKELDGHVL
841KCVKDQNGNH VVQKCIECVQ PQSLQFIIDA FKGQVFVLST HPYGCRVIQR ILEHCTAEQT
901LPILEELHQH TEQLVQDQYG NYVIQHVLEH GRPEDKSKIV SEIRGKVLAL SQHKFASNVV
961EKCVTHASRA ERALLIDEVC CQNDGPHSAL YTMMKDQYAN YVVQKMIDMA EPAQRKIIMH
1021KIRPHITTLR KYTYGKHILA KLEKYYLKNS PDLGPIGGPP NGML
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1064 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.