PUM2 Antibody
Catalog Number: LS-C752576-100
100 µg / $338.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PUM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77020-20
Catalog Number: LS-G77020
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Pumilio 2(PUM2) (NM_015317) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0235 | PUM2 | Pumilio homolog 2 (Drosophila) | PUMH2 | Pumilio (Drosphila) homolog 2 | Pumilio-2 | Pumilio homolog 2 | PUML2
- Origin Species
- Human
- NCBI GenBank
- NM_015317
- SwissProt
- Q8TB72
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 113.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW GASHHSMSQP61IMVQRRSGQG FHGNSEVNAI LSPRSESGGL GVSMVEYVLS SSPADKLDSR FRKGNFGTRD121AETDGPEKGD QKGKASPFEE DQNRDLKQGD DDDSKINGRG LPNGMDADCK DFNRTPGSRQ181ASPTEVVERL GPNTNPSEGL GPLPNPTANK PLVEEFSNPE TQNLDAMEQV GLESLQFDYP241GNQVPMDSSG ATVGLFDYNS QQQLFQRTNA LTVQQLTAAQ QQQYALAAAQ QPHIAGVFSA301GLAPAAFVPN PYIISAAPPG TDPYTAAGLA AAATLAGPAV VPPQYYGVPW GVYPANLFQQ361QAAAAANNTA SQQAASQAQP GQQQVLRAGA GQRPLTPNQG QQGQQAESLA AAAAANPTLA421FGQGLATGMP GYQVLAPTAY YDQTGALVVG PGARTGLGAP VRLMAPTPVL ISSAAAQAAA481AAAAGGTASS LTGSTNGLFR PIGTQPPQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS541HGPGQPGSTS LGFGSGNSLG AAIGSALSGF GSSVGSSASS SATRRESLST SSDLYKRSSS601SLAPIGQPFY NSLGFSSSPS PIGMPLPSQT PGHSLTPPPS LSSHGSSSSL HLGGLTNGSG661RYISAAPGAE AKYRSASSTS SLFSSSSQLF PPSRLRYNRS DIMPSGRSRL LEDFRNNRFP721NLQLRDLIGH IVEFSQDQHG SRFIQQKLER ATPAERQMVF NEILQAAYQL MTDVFGNYVI781QKFFEFGSLD QKLALATRIR GHVLPLALQM YGCRVIQKAL ESISSDQQSE MVKELDGHVL841KCVKDQNGNH VVQKCIECVQ PQSLQFIIDA FKGQVFVLST HPYGCRVIQR ILEHCTAEQT901LPILEELHQH TEQLVQDQYG NYVIQHVLEH GRPEDKSKIV SEIRGKVLAL SQHKFASNVV961EKCVTHASRA ERALLIDEVC CQNDGPHSAL YTMMKDQYAN YVVQKMIDMA EPAQRKIIMH1021KIRPHITTLR KYTYGKHILA KLEKYYLKNS PDLGPIGGPP NGMLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1064 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
