Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPRN2 / Phogrin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107602-100
Catalog Number: LS-G107602
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPRN2 (NM_130842), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ICAAR | Ia-2 beta | IA-2beta | PTPRP | R-PTP-N2 | Phogrin | Ptp-np | PTPRN2 | IAR | KIAA0387 | PTP NE-6 | Tyrosine phosphatase IA-2 beta
- Origin Species
- Human
- NCBI GenBank
- NM_002847
- SwissProt
- Q92932
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 109.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPPLPLLLL LLLLLPPRVL PAAPSSVPRG RQLPGRLDGV FGRCQKVPAM DFYRYEVSPV61ALQRLRVALQ KLSGTGFTWQ DDYTQYVMDQ ELADLPKTYL RRPEASSPAR PSKHSVGSER121RYSREGGAAL ANALRRHLPF LEALSQAPAS DVLARTHTAQ DRPPAEGDDR FSESILTYVA181HTSALTYPPG PRTQLREDLL PRTLGQLQPD ELSPKVDSGV DRHHLMAALS AYAAQRPPAP241PGEGSLEPQY LLRAPSRMPR PLLAPAAPQK WPSPLGDSED PSSTGDGARI HTLLKDLQRQ301PAEVRGLNGL ELDGMAELMA GLMQGVDHGV ARGSPGRAAL GESGEQADGP KATLRGDSFP361DDGVQDDDDR LYQEVHRLSA TLGGLLQDHG SRLLPGALPF ARPLDMERKK SEHPESSLSS421EEETAGVENV KSQTYSKDLL GQQPHSEPGA AAFGELQNQM PGPSKEEQSL PAGAQEALSD481GLQLEVQPSE EEARGYIVTD RDPLRPEEGR RLVEDVARLL QVPSSAFADV EVLGPAVTFK541VSANVQNVTT EDVEKATVDN KDKLEETSGL KILQTGVGSK SKLKFLPPQA EQEDSTKFIA601LTLVSLACIL GVLLASGLIY CLRHSSQHRL KEKLSGLGGD PGADATAAYQ ELCRQRMATR661PPDRPEGPHT SRISSVSSQF SDGPIPSPSA RSSASSWSEE PVQSNMDIST GHMILSYMED721HLKNKNRLEK EWEALCAYQA EPNSSFVAQR EENVPKNRSL AVLTYDHSRV LLKAENSHSH781SDYINASPIM DHDPRNPAYI ATQGPLPATV ADFWQMVWES GCVVIVMLTP LAENGVRQCY841HYWPDEGSNL YHIYEVNLVS EHIWCEDFLV RSFYLKNLQT NETRTVTQFH FLSWYDRGVP901SSSRSLLDFR RKVNKCYRGR SCPIIVHCSD GAGRSGTYVL IDMVLNKMAK GAKEIDIAAT961LEHLRDQRPG MVQTKEQFEF ALTAVAEEVN AILKALPQSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
998 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.