LSBio
Human PTPRN2 / Phogrin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PTPRN2 / Phogrin Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PTPRN2 antibodies →
$220.00each
Catalog Number: LS-G78418-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PTPRN2 (NM_130842) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ICAAR | Ia-2 beta | IA-2beta | PTPRP | R-PTP-N2 | Phogrin | Ptp-np | PTPRN2 | IAR | KIAA0387 | PTP NE-6 | Tyrosine phosphatase IA-2 beta
Origin Species
Human
NCBI GenBank
NM_002847
SwissProt
Q92932

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPPLPLLLL LLLLLPPRVL PAAPSSVPRG RQLPGRLDGV FGRCQKVPAM DFYRYEVSPV
61ALQRLRVALQ KLSGTGFTWQ DDYTQYVMDQ ELADLPKTYL RRPEASSPAR PSKHSVGSER
121RYSREGGAAL ANALRRHLPF LEALSQAPAS DVLARTHTAQ DRPPAEGDDR FSESILTYVA
181HTSALTYPPG PRTQLREDLL PRTLGQLQPD ELSPKVDSGV DRHHLMAALS AYAAQRPPAP
241PGEGSLEPQY LLRAPSRMPR PLLAPAAPQK WPSPLGDSED PSSTGDGARI HTLLKDLQRQ
301PAEVRGLNGL ELDGMAELMA GLMQGVDHGV ARGSPGRAAL GESGEQADGP KATLRGDSFP
361DDGVQDDDDR LYQEVHRLSA TLGGLLQDHG SRLLPGALPF ARPLDMERKK SEHPESSLSS
421EEETAGVENV KSQTYSKDLL GQQPHSEPGA AAFGELQNQM PGPSKEEQSL PAGAQEALSD
481GLQLEVQPSE EEARGYIVTD RDPLRPEEGR RLVEDVARLL QVPSSAFADV EVLGPAVTFK
541VSANVQNVTT EDVEKATVDN KDKLEETSGL KILQTGVGSK SKLKFLPPQA EQEDSTKFIA
601LTLVSLACIL GVLLASGLIY CLRHSSQHRL KEKLSGLGGD PGADATAAYQ ELCRQRMATR
661PPDRPEGPHT SRISSVSSQF SDGPIPSPSA RSSASSWSEE PVQSNMDIST GHMILSYMED
721HLKNKNRLEK EWEALCAYQA EPNSSFVAQR EENVPKNRSL AVLTYDHSRV LLKAENSHSH
781SDYINASPIM DHDPRNPAYI ATQGPLPATV ADFWQMVWES GCVVIVMLTP LAENGVRQCY
841HYWPDEGSNL YHIYEVNLVS EHIWCEDFLV RSFYLKNLQT NETRTVTQFH FLSWYDRGVP
901SSSRSLLDFR RKVNKCYRGR SCPIIVHCSD GAGRSGTYVL IDMVLNKMAK GAKEIDIAAT
961LEHLRDQRPG MVQTKEQFEF ALTAVAEEVN AILKALPQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

998 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.