Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPRM / PTP Mu Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124113-100
Catalog Number: LS-G124113
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RPTP mu(PTPRM) (NM_001105244), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HR-PTPu | PTP mu | PTPRL1 | PTPRM | RPTPM | Ptpmu | R-PTP-MU | RPTPU
- Origin Species
- Human
- NCBI GenBank
- NM_002845
- SwissProt
- P28827
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 165.06 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRGLGTCLAT LAGLLLTAAG ETFSGGCLFD EPYSTCGYSQ SEGDDFNWEQ VNTLTKPTSD61PWMPSGSFML VNASGRPEGQ RAHLLLPQLK ENDTHCIDFH YFVSSKSNSP PGLLNVYVKV121NNGPLGNPIW NISGDPTRTW NRAELAISTF WPNFYQVIFE VITSGHQGYL AIDEVKVLGH181PCTRTPHFLR IQNVEVNAGQ FATFQCSAIG RTVAGDRLWL QGIDVRDAPL KEIKVTSSRR241FIASFNVVNT TKRDAGKYRC MIRTEGGVGI SNYAELVVKE PPVPIAPPQL ASVGATYLWI301QLNANSINGD GPIVAREVEY CTASGSWNDR QPVDSTSYKI GHLDPDTEYE ISVLLTRPGE361GGTGSPGPAL RTRTKCADPM RGPRKLEVVE VKSRQITIRW EPFGYNVTRC HSYNLTVHYC421YQVGGQEQVR EEVSWDTENS HPQHTITNLS PYTNVSVKLI LMNPEGRKES QELIVQTDED481LPGAVPTESI QGSTFEEKIF LQWREPTQTY GVITLYEITY KAVSSFDPEI DLSNQSGRVS541KLGNETHFLF FGLYPGTTYS FTIRASTAKG FGPPATNQFT TKISAPSMPA YELETPLNQT601DNTVTVMLKP AHSRGAPVSV YQIVVEEERP RRTKKTTEIL KCYPVPIHFQ NASLLNSQYY661FAAEFPADSL QAAQPFTIGD NKTYNGYWNT PLLPYKSYRI YFQAASRANG ETKIDCVQVA721TKGAATPKPV PEPEKQTDHT VKIAGVIAGI LLFVIIFLGV VLVMKKRKLA KKRKETMSST781RQEMTVMVNS MDKSYAEQGT NCDEAFSFMD THNLNGRSVS SPSSFTMKTN TLSTSVPNSY841YPDPFVPTAI LVPINDETHT MASDTSSLVQ SHTYKKREPA DVPYQTGQLH PAIRVADLLQ901HITQMKCAEG YGFKEEYESF FEGQSAPWDS AKKDENRMKN RYGNIIAYDH SRVRLQTIEG961DTNSDYINGN YIDGYHRPNH YIATQGPMQE TIYDFWRMVW HENTASIIMV TNLVEVGRVK1021CCKYWPDDTE IYKDIKVTLI ETELLAEYVI RTFAVEKRGV HEIREIRQFH FTGWPDHGVP1081YHATGLLGFV RQVKSKSPPS AGPLVVHCSA GAGRTGCFIV IDIMLDMAER EGVVDIYNCV1141RELRSRRVNM VQTEEQYVFI HDAILEACLC GDTSVPASQV RSLYYDMNKL DPQTNSSQIK1201EEFRTLNMVT PTLRVEDCSI ALLPRNHEKN RCMDILPPDR CLPFLITIDG ESSNYINAAL1261MDSYKQPSAF IVTQHPLPNT VKDFWRLVLD YHCTSVVMLN DVDPAQLCPQ YWPENGVHRH1321GPIQVEFVSA DLEEDIISRI FRIYNAARPQ DGYRMVQQFQ FLGWPMYRDT PVSKRSFLKL1381IRQVDKWQEE YNGGEGRTVV HCLNGGGRSG TFCAISIVCE MLRHQRTVDV FHAVKTLRNN1441KPNMVDLLDQ YKFCYEVALE YLNSGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.