LSBio
Human PTPRM / PTP Mu Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PTPRM / PTP Mu Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PTPRM antibodies →
$220.00each
Catalog Number: LS-G78415-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RPTP mu(PTPRM) (NM_001105244) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HR-PTPu | PTP mu | PTPRL1 | PTPRM | RPTPM | Ptpmu | R-PTP-MU | RPTPU
Origin Species
Human
NCBI GenBank
NM_002845
SwissProt
P28827

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
165.06 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGLGTCLAT LAGLLLTAAG ETFSGGCLFD EPYSTCGYSQ SEGDDFNWEQ VNTLTKPTSD
61PWMPSGSFML VNASGRPEGQ RAHLLLPQLK ENDTHCIDFH YFVSSKSNSP PGLLNVYVKV
121NNGPLGNPIW NISGDPTRTW NRAELAISTF WPNFYQVIFE VITSGHQGYL AIDEVKVLGH
181PCTRTPHFLR IQNVEVNAGQ FATFQCSAIG RTVAGDRLWL QGIDVRDAPL KEIKVTSSRR
241FIASFNVVNT TKRDAGKYRC MIRTEGGVGI SNYAELVVKE PPVPIAPPQL ASVGATYLWI
301QLNANSINGD GPIVAREVEY CTASGSWNDR QPVDSTSYKI GHLDPDTEYE ISVLLTRPGE
361GGTGSPGPAL RTRTKCADPM RGPRKLEVVE VKSRQITIRW EPFGYNVTRC HSYNLTVHYC
421YQVGGQEQVR EEVSWDTENS HPQHTITNLS PYTNVSVKLI LMNPEGRKES QELIVQTDED
481LPGAVPTESI QGSTFEEKIF LQWREPTQTY GVITLYEITY KAVSSFDPEI DLSNQSGRVS
541KLGNETHFLF FGLYPGTTYS FTIRASTAKG FGPPATNQFT TKISAPSMPA YELETPLNQT
601DNTVTVMLKP AHSRGAPVSV YQIVVEEERP RRTKKTTEIL KCYPVPIHFQ NASLLNSQYY
661FAAEFPADSL QAAQPFTIGD NKTYNGYWNT PLLPYKSYRI YFQAASRANG ETKIDCVQVA
721TKGAATPKPV PEPEKQTDHT VKIAGVIAGI LLFVIIFLGV VLVMKKRKLA KKRKETMSST
781RQEMTVMVNS MDKSYAEQGT NCDEAFSFMD THNLNGRSVS SPSSFTMKTN TLSTSVPNSY
841YPDPFVPTAI LVPINDETHT MASDTSSLVQ SHTYKKREPA DVPYQTGQLH PAIRVADLLQ
901HITQMKCAEG YGFKEEYESF FEGQSAPWDS AKKDENRMKN RYGNIIAYDH SRVRLQTIEG
961DTNSDYINGN YIDGYHRPNH YIATQGPMQE TIYDFWRMVW HENTASIIMV TNLVEVGRVK
1021CCKYWPDDTE IYKDIKVTLI ETELLAEYVI RTFAVEKRGV HEIREIRQFH FTGWPDHGVP
1081YHATGLLGFV RQVKSKSPPS AGPLVVHCSA GAGRTGCFIV IDIMLDMAER EGVVDIYNCV
1141RELRSRRVNM VQTEEQYVFI HDAILEACLC GDTSVPASQV RSLYYDMNKL DPQTNSSQIK
1201EEFRTLNMVT PTLRVEDCSI ALLPRNHEKN RCMDILPPDR CLPFLITIDG ESSNYINAAL
1261MDSYKQPSAF IVTQHPLPNT VKDFWRLVLD YHCTSVVMLN DVDPAQLCPQ YWPENGVHRH
1321GPIQVEFVSA DLEEDIISRI FRIYNAARPQ DGYRMVQQFQ FLGWPMYRDT PVSKRSFLKL
1381IRQVDKWQEE YNGGEGRTVV HCLNGGGRSG TFCAISIVCE MLRHQRTVDV FHAVKTLRNN
1441KPNMVDLLDQ YKFCYEVALE YLNSG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1465 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.