Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPN21 / PTPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114902-100
Catalog Number: LS-G114902
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPD1(PTPN21) (NM_007039), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PTPN21 | PTP2E | PTPRL10 | PTPD1
- Origin Species
- Human
- NCBI GenBank
- NM_007039
- SwissProt
- Q16825
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLPFGLKLK RTRRYTVSSK SCLVARIQLL NNEFVEFTLS VESTGQESLE AVAQRLELRE61VTYFSLWYYN KQNQRRWVDL EKPLKKQLDK YALEPTVYFG VVFYVPSVSQ LQQEITRYQY121YLQLKKDILE GSIPCTLEQA IQLAGLAVQA DFGDFDQYES QDFLQKFALF PVGWLQDEKV181LEEATQKVAL LHQKYRGLTA PDAEMLYMQE VERMDGYGEE SYPAKDSQGS DISIGACLEG241IFVKHKNGRH PVVFRWHDIA NMSHNKSFFA LELANKEETI QFQTEDMETA KYIWRLCVAR301HKFYRLNQCN LQTQTVTVNP IRRRSSSRMS LPKPQPYVMP PPPQLHYNGH YTEPYASSQD361NLFVPNQNGY YCHSQTSLDR AQIDLNGRIR NGSVYSAHST NSLNNPQPYL QPSPMSSNPS421ITGSDVMRPD YLPSHRHSAV IPPSYRPTPD YETVMKQLNR GLVHAERQSH SLRNLNIGSS481YAYSRPAALV YSQPEIREHA QLPSPAAAHC PFSLSYSFHS PSPYPYPAER RPVVGAVSVP541ELTNAQLQAQ DYPSPNIMRT QVYRPPPPYP PPRPANSTPD LSRHLYISSS NPDLITRRVH601HSVQTFQEDS LPVAHSLQEV SEPLTAARHA QLHKRNSIEV AGLSHGLEGL RLKERTLSAS661AAEVAPRAVS VGSQPSVFTE RTQREGPEEA EGLRYGHKKS LSDATMLIHS SEEEEDEDFE721EESGARAPPA RAREPRPGLA QDPPGCPRVL LAGPLHILEP KAHVPDAEKR MMDSSPVRTT781AEAQRPWRDG LLMPSMSESD LTTSGRYRAR RDSLKKRPVS DLLSGKKNIV EGLPPLGGMK841KTRVDAKKIG PLKLAALNGL SLSRVPLPDE GKEVATRATN DERCKILEQR LEQGMVFTEY901ERILKKRLVD GECSTARLPE NAERNRFQDV LPYDDVRVEL VPTKENNTGY INASHIKVSV961SGIEWDYIAT QGPLQNTCQD FWQMVWEQGI AIIAMVTAEE EGGREKSFRY WPRLGSRHNT1021VTYGRFKITT RFRTDSGCYA TTGLKMKHLL TGQERTVWHL QYTDWPEHGC PEDLKGFLSY1081LEEIQSVRRH TNSTSDPQSP NPPLLVHCSA GVGRTGVVIL SEIMIACLEH NEVLDIPRVL1141DMLRQQRMML VQTLCQYTFV YRVLIQFLKS SRLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
1174 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.