LSBio
Human PTPN21 / PTPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PTPN21 / PTPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PTPN21 antibodies →
$220.00each
Catalog Number: LS-G78404-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PTPD1(PTPN21) (NM_007039) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PTPN21 | PTP2E | PTPRL10 | PTPD1
Origin Species
Human
NCBI GenBank
NM_007039
SwissProt
Q16825

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLPFGLKLK RTRRYTVSSK SCLVARIQLL NNEFVEFTLS VESTGQESLE AVAQRLELRE
61VTYFSLWYYN KQNQRRWVDL EKPLKKQLDK YALEPTVYFG VVFYVPSVSQ LQQEITRYQY
121YLQLKKDILE GSIPCTLEQA IQLAGLAVQA DFGDFDQYES QDFLQKFALF PVGWLQDEKV
181LEEATQKVAL LHQKYRGLTA PDAEMLYMQE VERMDGYGEE SYPAKDSQGS DISIGACLEG
241IFVKHKNGRH PVVFRWHDIA NMSHNKSFFA LELANKEETI QFQTEDMETA KYIWRLCVAR
301HKFYRLNQCN LQTQTVTVNP IRRRSSSRMS LPKPQPYVMP PPPQLHYNGH YTEPYASSQD
361NLFVPNQNGY YCHSQTSLDR AQIDLNGRIR NGSVYSAHST NSLNNPQPYL QPSPMSSNPS
421ITGSDVMRPD YLPSHRHSAV IPPSYRPTPD YETVMKQLNR GLVHAERQSH SLRNLNIGSS
481YAYSRPAALV YSQPEIREHA QLPSPAAAHC PFSLSYSFHS PSPYPYPAER RPVVGAVSVP
541ELTNAQLQAQ DYPSPNIMRT QVYRPPPPYP PPRPANSTPD LSRHLYISSS NPDLITRRVH
601HSVQTFQEDS LPVAHSLQEV SEPLTAARHA QLHKRNSIEV AGLSHGLEGL RLKERTLSAS
661AAEVAPRAVS VGSQPSVFTE RTQREGPEEA EGLRYGHKKS LSDATMLIHS SEEEEDEDFE
721EESGARAPPA RAREPRPGLA QDPPGCPRVL LAGPLHILEP KAHVPDAEKR MMDSSPVRTT
781AEAQRPWRDG LLMPSMSESD LTTSGRYRAR RDSLKKRPVS DLLSGKKNIV EGLPPLGGMK
841KTRVDAKKIG PLKLAALNGL SLSRVPLPDE GKEVATRATN DERCKILEQR LEQGMVFTEY
901ERILKKRLVD GECSTARLPE NAERNRFQDV LPYDDVRVEL VPTKENNTGY INASHIKVSV
961SGIEWDYIAT QGPLQNTCQD FWQMVWEQGI AIIAMVTAEE EGGREKSFRY WPRLGSRHNT
1021VTYGRFKITT RFRTDSGCYA TTGLKMKHLL TGQERTVWHL QYTDWPEHGC PEDLKGFLSY
1081LEEIQSVRRH TNSTSDPQSP NPPLLVHCSA GVGRTGVVIL SEIMIACLEH NEVLDIPRVL
1141DMLRQQRMML VQTLCQYTFV YRVLIQFLKS SRLI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1174 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.