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PTPDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPDC1 antibodies →
$504.00each
Catalog Number: LS-G104630-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPDC1 (NM_177995), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_152422

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGVLPQNE QPYSTLVNNS ECVANMKGNL ERPTPKYTKV GERLRHVIPG HMACSMACGG
61RACKYENPAR WSEQEQAIKG VYSSWVTDNI LAMARPSSEL LEKYHIIDQF LSHGIKTIIN
121LQRPGEHASC GNPLEQESGF TYLPEAFMEA GIYFYNFGWK DYGVASLTTI LDMVKVMTFA
181LQEGKVAIHC HAGLGRTGVL IACYLVFATR MTADQAIIFV RAKRPNSIQT RGQLLCVREF
241TQFLTPLRNI FSCCDPKAHA VTLPQYLIRQ RHLLHGYEAR LLKHVPKIIH LVCKLLLDLA
301ENRPVMMKDV SEGPGLSAEI EKTMSEMVTM QLDKELLRHD SDFSNPPNPT AVAADFDNRG
361MIFSNEQQFD PLWKRRNVEC LQPLTHLKRR LSYSDSDLKR AENLLEQGET PQTVPAQILV
421GHKPRQQKLI SHCYIPQSPE PDLHKEALVR STLSFWSQSK FGGLEGLKDN GSPIFHGRII
481PKEAQQSGAF SADVSGSHSP GEPVSPSFAN VHKDPNPAHQ QVSHCQCKTH GVGSPGSVRQ
541NSRTPRSPLD CGSSPKAQFL VEHETQDSKD LSEAASHSAL QSELSAEARR ILAAKALANL
601NESVEKEELK RKVEMWQKEL NSRDGAWERI CGERDPFILC SLMWSWVEQL KEPVITKEDV
661DMLVDRRADA AEALFLLEKG QHQTILCVLH CIVNLQTIPV DVEEAFLAHA IKAFTKVNFD
721SENGPTVYNT LKKIFKHTLE EKRKMTKDGP KPGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

754 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.