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PTPDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPDC1 antibodies →
$725.00each
Catalog Number: LS-G102351-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPDC1 (NM_152422), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_152422

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQVQDATRRP SAVRFLSSFL QGRRHSTSDP VLRLQQARRG SGLGSGSATK LLSSSSLQVM
61VAVSSVSHAE GNPTFPERKR NLERPTPKYT KVGERLRHVI PGHMACSMAC GGRACKYENP
121ARWSEQEQAI KGVYSSWVTD NILAMARPSS ELLEKYHIID QFLSHGIKTI INLQRPGEHA
181SCGNPLEQES GFTYLPEAFM EAGIYFYNFG WKDYGVASLT TILDMVKVMT FALQEGKVAI
241HCHAGLGRTG VLIACYLVFA TRMTADQAII FVRAKRPNSI QTRGQLLCVR EFTQFLTPLR
301NIFSCCDPKA HAVTLPQYLI RQRHLLHGYE ARLLKHVPKI IHLVCKLLLD LAENRPVMMK
361DVSEGPGLSA EIEKTMSEMV TMQLDKELLR HDSDVSNPPN PTAVAADFDN RGMIFSNEQQ
421FDPLWKRRNV ECLQPLTHLK RRLSYSDSDL KRAENLLEQG ETPQTVPAQI LVGHKPRQQK
481LISHCYIPQS PEPDLHKEAL VRSTLSFWSQ SKFGGLEGLK DNGSPIFHGR IIPKEAQQSG
541AFSADVSGSH SPGEPVSPSF ANVHKDPNPA HQQVSHCQCK THGVGSPGSV RQNSRTPRSP
601LDCGSSPKAQ FLVEHETQDS KDLSEAASHS ALQSELSAEA RRILAAKALA NLNESVEKEE
661LKRKVEMWQK ELNSRDGAWE RICGERDPFI LCSLMWSWVE QLKEPVITKE DVDMLVDRRA
721DAAEALFLLE KGQHQTILCV LHCIVNLQTI PVDVEEAFLA HAIKAFTKVN FDSENGPTVY
781NTLKKIFKHT LEEKRKMTKD GPKPGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

806 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.