Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTGER3 / EP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103596-100
Catalog Number: LS-G103596
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTGER3 (NM_198715), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ep3 prostanoid receptor | Ep3 receptor | EP3-I | EP3e | EP3-II | EP3-III | EP3-IV | PGE receptor | EP3 subtype | Prostaglandin receptor (PGE-2) | Prostaglandin ep3 receptor | Prostanoid EP3 receptor | PTGER3 | PGE receptor EP3 subtype | Pge2 receptor ep3 | PGE2 receptor EP3 subtype | Prostaglandin E receptor 3 | Prostaglandin e2 receptor ep3 | EP3 | Ep3 subtype pge2 receptor | PGE2-R | Prostaglandin E receptor EP3
- Origin Species
- Human
- NCBI GenBank
- NM_000957
- SwissProt
- P43115
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKETRGYGGD APFCTRLNHS YTGMWAPERS AEARGNLTRP PGSGEDCGSV SVAFPITMLL61TGFVGNALAM LLVSRSYRRR ESKRKKSFLL CIGWLALTDL VGQLLTTPVV IVVYLSKQRW121EHIDPSGRLC TFFGLTMTVF GLSSLFIASA MAVERALAIR APHWYASHMK TRATRAVLLG181VWLAVLAFAL LPVLGVGQYT VQWPGTWCFI STGRGGNGTS SSHNWGNLFF ASAFAFLGLL241ALTVTFSCNL ATIKALVSRC RAKATASQSS AQWGRITTET AIQLMGIMCV LSVCWSPLLI301MMLKMIFNQT SVEHCKTHTE KQKECNFFLI AVRLASLNQI LDPWVYLLLR KILLRKFCQV361ANAVSSCSND GQKGQPISLS NEIIQTEASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
388 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.