Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRMT4 / CARM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103517-100
Catalog Number: LS-G103517
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRMT4(CARM1) (NM_199141), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CARM1 | PRMT4
- Origin Species
- Human
- NCBI GenBank
- NM_199141
- SwissProt
- Q86X55
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAAAVGP GAGGAGSAVP GGAGPCATVS VFPGARLLTI GDANGEIQRH AEQQALRLEV61RAGPDSAGIA LYSHEDVCVF KCSVSRETEC SRVGKQSFII TLGCNSVLIQ FATPNDFCSF121YNILKTCRGH TLERSVFSER TEESSAVQYF QFYGYLSQQQ NMMQDYVRTG TYQRAILQNH181TDFKDKIVLD VXLWLWDPVV FCRPSWSTEN LRGXRPAPWP STXEVLVKSN NLTDRIVVIP241GKVXGXCXXP XXQVDIIXLG AHGLHALQRX ACWRATSTPX KYLKPSGNMF PTIGDVHLAP301FTDEQLYMEQ FTKANFWYQP SFHGVDLSAL RGAAVDEYFR QPVVDTFDIR ILMAKSVKYT361VNFLEAKEGD LHRIEIPFKF HMLHSGLVHG LAFWFDVAFI GSIMTVWLST APTEPLTHWY421QVRCLFQSPL FAKAGDTLSG TCLLIANKRQ SYDISIVAQV DQTGSKSSNL LDLKNPFFRY481TGTTPSPPPG SHYTSPSENM WNTGSTYNLS SGMAVAGMPT AYDLSSVIAS GSSVGHNNLI541PLANTGIVNH THSRMGSIMS TGIVQGSSGA QGSGGGSTSA HYAVNSQFTM GGPAISMASP601MSIPTNTMHY GSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
612 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.