PRMT4 / CARM1 Antibody
Catalog Number: LS-C332011
2 sizes / from $293.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRMT4 / CARM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87776-20
Catalog Number: LS-G87776
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRMT4(CARM1) (NM_199141) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CARM1 | PRMT4
- Origin Species
- Human
- NCBI GenBank
- NM_199141
- SwissProt
- Q86X55
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAAAVGP GAGGAGSAVP GGAGPCATVS VFPGARLLTI GDANGEIQRH AEQQALRLEV61RAGPDSAGIA LYSHEDVCVF KCSVSRETEC SRVGKQSFII TLGCNSVLIQ FATPNDFCSF121YNILKTCRGH TLERSVFSER TEESSAVQYF QFYGYLSQQQ NMMQDYVRTG TYQRAILQNH181TDFKDKIVLD VXLWLWDPVV FCRPSWSTEN LRGXRPAPWP STXEVLVKSN NLTDRIVVIP241GKVXGXCXXP XXQVDIIXLG AHGLHALQRX ACWRATSTPX KYLKPSGNMF PTIGDVHLAP301FTDEQLYMEQ FTKANFWYQP SFHGVDLSAL RGAAVDEYFR QPVVDTFDIR ILMAKSVKYT361VNFLEAKEGD LHRIEIPFKF HMLHSGLVHG LAFWFDVAFI GSIMTVWLST APTEPLTHWY421QVRCLFQSPL FAKAGDTLSG TCLLIANKRQ SYDISIVAQV DQTGSKSSNL LDLKNPFFRY481TGTTPSPPPG SHYTSPSENM WNTGSTYNLS SGMAVAGMPT AYDLSSVIAS GSSVGHNNLI541PLANTGIVNH THSRMGSIMS TGIVQGSSGA QGSGGGSTSA HYAVNSQFTM GGPAISMASP601MSIPTNTMHY GSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
612 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
