Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRKAG2 / AMPK Gamma 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120356-100
Catalog Number: LS-G120356
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRKAG2 (NM_001040633), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ampk gamma 2 | AAKG | AAKG2 | AAMPK Gamma 2 | AMPK subunit gamma-2 | AMPK gamma2 | CMH6 | WPWS | H91620p | PRKAG2
- Origin Species
- Human
- NCBI GenBank
- NM_016203
- SwissProt
- Q9UGJ0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLLDGDLEG SGKHSSRKVD SPFGPGSPSK GFFSRGPQPR PSSPMSAPVR PKTSPGSPKT61VFPFSYQESP PRSPRRMSFS GIFRSSSKES SPNSNPATSP GGIRFFSRSR KTSGLSSSPS121TPTQVTKQHT FPLESYKHEP ERLENRIYAS SSPPDTGQRF CPSSFQSPTR PPLASPTHYA181PSKAAALAAA LGPAEAGMLE KLEFEDEAVE DSESGVYMRF MRSHKCYDIV PTSSKLVVFD241TTLQVKKAFF ALVANGVRAA PLWESKKQSF VGMLTITDFI NILHRYYKSP MVQIYELEEH301KIETWRELYL QETFKPLVNI SPDASLFDAV YSLIKNKIHR LPVIDPISGN ALYILTHKRI361LKFLQLFMSD MPKPAFMKQN LDELGIGTYH NIAFIHPDTP IIKALNIFVE RRISALPVVD421ESGKVVDIYS KFDVINLAAE KTYNNLDITV TQALQHRSQY FEGVVKCNKL EILETIVDRI481VRAEVHRLVV VNEADSIVGI ISLSDILQAL ILTPAGAKQK ETETETRTRPLEQKLISEEDLAANDILDYKDDDDKV
525 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.