Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRKAG2 / AMPK Gamma 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101400-100
Catalog Number: LS-G101400
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRKAG2 (NM_016203), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ampk gamma 2 | AAKG | AAKG2 | AAMPK Gamma 2 | AMPK subunit gamma-2 | AMPK gamma2 | CMH6 | WPWS | H91620p | PRKAG2
- Origin Species
- Human
- NCBI GenBank
- NM_016203
- SwissProt
- Q9UGJ0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSAVMDTKK KKDVSSPGGS GGKKNASQKR RSLRVHIPDL SSFAMPLLDG DLEGSGKHSS61RKVDSPFGPG SPSKGFFSRG PQPRPSSPMS APVRPKTSPG SPKTVFPFSY QESPPRSPRR121MSFSGIFRSS SKESSPNSNP ATSPGGIRFF SRSRKTSGLS SSPSTPTQVT KQHTFPLESY181KHEPERLENR IYASSSPPDT GQRFCPSSFQ SPTRPPLASP THYAPSKAAA LAAALGPAEA241GMLEKLEFED EVEDSESGVY MRFMRSHKCY DIVPTSSKLV VFDTTLQVKK AFFALVANGV301RAAPLWESKK QSFVGMLTIT DFINILHRYY KSPMVQIYEL EEHKIETWRE LYLQETFKPL361VNISPDASLF DAVYSLIKNK IHRLPVIDPI SGNALYILTH KRILKFLQLF MSDMPKPAFM421KQNLDELGIG TYHNIAFIHP DTPIIKALNI FVERRISALP VVDESGKVVD IYSKFDVINL481AAEKTYNNLD ITVTQALQHR SQYFEGVVKC NKLEILETIV DRIVRAEVHR LVVVNEADSI541VGIISLSDIL QALILTPAGA KQKETETETRTRPLEQKLISEEDLAANDILDYKDDDDKV
568 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.