Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRICKLE3 / LMO6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115499-100
Catalog Number: LS-G115499
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRICKLE3 (NM_006150), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LMO6 | LIM domain only 6 | Triple LIM domain protein 6 | LIM domain only protein 6 | LMO-6 | Prickle homolog 3 (Drosophila) | Prickle-like protein 3 | PRICKLE3
- Origin Species
- Human
- NCBI GenBank
- NM_006150
- SwissProt
- O43900
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFARGSRRRR SGRAPPEAED PDRGQPCNSC REQCPGFLLH GWRKICQHCK CPREEHAVHA61VPVDLERIMC RLISDFQRHS ISDDDSGCAS EEYAWVPPGL KPEQVYQFFS CLPEDKVPYV121NSPGEKYRIK QLLHQLPPHD SEAQYCTALE EEEKKELRAF SQQRKRENLG RGIVRIFPVT181ITGAICEECG KQIGGGDIAV FASRAGLGAC WHPQCFVCTT CQELLVDLIY FYHVGKVYCG241RHHAECLRPR CQACDEIIFS PECTEAEGRH WHMDHFCCFE CEASLGGQRY VMRQSRPHCC301ACYEARHAEY CDGCGEHIGL DQGQMAYEGQ HWHASDRCFC CSRCGRALLG RPFLPRRGLI361FCSRACSLGS EPTAPGPSRR SWSAGPVTAP LAASTASFSA VKGASETTTK GTSTELAPAT421GPEEPSRFLR GAPHRHSMPE LGLRSVPEPP PESPGQPNLR PDDSAFGRQS TPRVSFRDPL481VSEGGPRRTL SAPPAQRRRP RSPPPRAPSR RRHHHHNHHH HHNRHPSRRR HYQCDAGSGS541DSESCSSSPS SSSSESSEDD GFFLGERIPL PPHLCRPMPA QDTAMETFNS PSLSLPRDSR601AGMPRQARDK NCIVASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
615 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.