Human PRICKLE3 / LMO6 Protein (Recombinant GST)
Catalog Number: LS-G29210
2 sizes / from $489.00
Expression System Tobacco / Plant-Based Expression
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRICKLE3 / LMO6 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G89479-20
Catalog Number: LS-G89479
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRICKLE3 (NM_006150) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LMO6 | LIM domain only 6 | Triple LIM domain protein 6 | LIM domain only protein 6 | LMO-6 | Prickle homolog 3 (Drosophila) | Prickle-like protein 3 | PRICKLE3
- Origin Species
- Human
- NCBI GenBank
- NM_006150
- SwissProt
- O43900
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFARGSRRRR SGRAPPEAED PDRGQPCNSC REQCPGFLLH GWRKICQHCK CPREEHAVHA61VPVDLERIMC RLISDFQRHS ISDDDSGCAS EEYAWVPPGL KPEQVYQFFS CLPEDKVPYV121NSPGEKYRIK QLLHQLPPHD SEAQYCTALE EEEKKELRAF SQQRKRENLG RGIVRIFPVT181ITGAICEECG KQIGGGDIAV FASRAGLGAC WHPQCFVCTT CQELLVDLIY FYHVGKVYCG241RHHAECLRPR CQACDEIIFS PECTEAEGRH WHMDHFCCFE CEASLGGQRY VMRQSRPHCC301ACYEARHAEY CDGCGEHIGL DQGQMAYEGQ HWHASDRCFC CSRCGRALLG RPFLPRRGLI361FCSRACSLGS EPTAPGPSRR SWSAGPVTAP LAASTASFSA VKGASETTTK GTSTELAPAT421GPEEPSRFLR GAPHRHSMPE LGLRSVPEPP PESPGQPNLR PDDSAFGRQS TPRVSFRDPL481VSEGGPRRTL SAPPAQRRRP RSPPPRAPSR RRHHHHNHHH HHNRHPSRRR HYQCDAGSGS541DSESCSSSPS SSSSESSEDD GFFLGERIPL PPHLCRPMPA QDTAMETFNS PSLSLPRDSR601AGMPRQARDK NCIVASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
615 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
