Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRDM10 / TRIS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103333-100
Catalog Number: LS-G103333
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRDM10 (NM_199437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1231 | PRDM10 | Tristanin | PFM7 | PR domain containing 10 | PR-domain family member 7 | TRIS
- Origin Species
- Human
- NCBI GenBank
- NM_020228
- SwissProt
- Q9NQV6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSKDESSHV WPTSAEHEQN AAQVHFVPDT GTVAQIVYTD DQVRPSQQVV YTADGASYTS61VDGPEHTLVY IHPVEAAQTL FTDPGQVAYV QQDATAQQAS LPVHNQVLPS IESVDGSDPL121ATLQTPLGRL EAKEEEDEDE DEDTEEDEEE DGEDTDLDDW EPDPPRPFDP HDLWCEECNN181AHASVCPKHG PLHPIPNRPV LTRARASLPL VLYIDRFLGG VFSKRRIPKR TQFGPVEGPL241VRGSELKDCY IHLKVSLDKG DRKERDLHED LWFELSDETL CNWMMFVRPA QNHLEQNLVA301YQYGHHVYYT TIKNVEPKQE LKVWYAASYA EFVNQKIHDI SEEERKVLRE QEKNWPCYEC361NRRFISSEQL QQHLNSHDEK LDVFSRTRGR GRGRGKRRFG PGRRPGRPPK FIRLEITSEN421GEKSDDGTQD LLHFPTKEQF DEAEPATLNG LDQPEQTTIP IPQLPQETQS SLEHEPETHT481LHLQPQHEES VVPTQSTLTA DDMRRAKRIR NAALQHLFIR KSFRPFKCLQ CGKAFREKDK541LDQHLRFHGR EGNCPLTCDL CNKGFISSTS LESHMKLHSD QKTYSCIFCP ESFDRLDLLK601DHVAIHINDG YFTCPTCKKR FPDFIQVKKH VRSFHSEKIY QCTECDKAFC RPDKLRLHML661RHSDRKDFLC STCGKQFKRK DKLREHMQRM HNPEREAKKA DRISRSKTFK PRITSTDYDS721FTFKCRLCMM GFRRRGMLVN HLSKRHPDMK IEEVPELTLP IIKPNRDYFC QYCDKVYKSA781SKRKAHILKN HPGAELPPSI RKLRPAGPGE PDPMLSTHTQ LTGTIATPPV CCPHCSKQYS841SKTKMVQHIR KKHPEFAQLS NTIHTPLTTA VISATPAVLT TDSATGETVV TTDLLTQAMT901ELSQTLTTDY RTPQGDYQRI QYIPVSQSAS GLQQPQHIQL QVVQVASATS PHQSQQSTVD961VGQLHDPQPY PQHAIQVQHI QVSEPTASAP SSAQVSGQPL SPSAQQAQQG LSPSHIQGSS1021STQGQALQQQ QQQQQNSSVQ HTYLPSAWNS FRGYSSEIQM MTLPPGQFVI TDSGVATPVT1081TGQVKAVTSG HYVLSESQSE LEEKQTSALS GGVQVEPPAH SDSLDPQTNS QQQTTQYIIT1141TTTNGNGSSE VHITKPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1156 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.