LSBio
Human PRDM10 / TRIS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PRDM10 / TRIS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PRDM10 antibodies →
$153.00each
Catalog Number: LS-G91213-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRDM10 (NM_199437) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1231 | PRDM10 | Tristanin | PFM7 | PR domain containing 10 | PR-domain family member 7 | TRIS
Origin Species
Human
NCBI GenBank
NM_020228
SwissProt
Q9NQV6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSKDESSHV WPTSAEHEQN AAQVHFVPDT GTVAQIVYTD DQVRPSQQVV YTADGASYTS
61VDGPEHTLVY IHPVEAAQTL FTDPGQVAYV QQDATAQQAS LPVHNQVLPS IESVDGSDPL
121ATLQTPLGRL EAKEEEDEDE DEDTEEDEEE DGEDTDLDDW EPDPPRPFDP HDLWCEECNN
181AHASVCPKHG PLHPIPNRPV LTRARASLPL VLYIDRFLGG VFSKRRIPKR TQFGPVEGPL
241VRGSELKDCY IHLKVSLDKG DRKERDLHED LWFELSDETL CNWMMFVRPA QNHLEQNLVA
301YQYGHHVYYT TIKNVEPKQE LKVWYAASYA EFVNQKIHDI SEEERKVLRE QEKNWPCYEC
361NRRFISSEQL QQHLNSHDEK LDVFSRTRGR GRGRGKRRFG PGRRPGRPPK FIRLEITSEN
421GEKSDDGTQD LLHFPTKEQF DEAEPATLNG LDQPEQTTIP IPQLPQETQS SLEHEPETHT
481LHLQPQHEES VVPTQSTLTA DDMRRAKRIR NAALQHLFIR KSFRPFKCLQ CGKAFREKDK
541LDQHLRFHGR EGNCPLTCDL CNKGFISSTS LESHMKLHSD QKTYSCIFCP ESFDRLDLLK
601DHVAIHINDG YFTCPTCKKR FPDFIQVKKH VRSFHSEKIY QCTECDKAFC RPDKLRLHML
661RHSDRKDFLC STCGKQFKRK DKLREHMQRM HNPEREAKKA DRISRSKTFK PRITSTDYDS
721FTFKCRLCMM GFRRRGMLVN HLSKRHPDMK IEEVPELTLP IIKPNRDYFC QYCDKVYKSA
781SKRKAHILKN HPGAELPPSI RKLRPAGPGE PDPMLSTHTQ LTGTIATPPV CCPHCSKQYS
841SKTKMVQHIR KKHPEFAQLS NTIHTPLTTA VISATPAVLT TDSATGETVV TTDLLTQAMT
901ELSQTLTTDY RTPQGDYQRI QYIPVSQSAS GLQQPQHIQL QVVQVASATS PHQSQQSTVD
961VGQLHDPQPY PQHAIQVQHI QVSEPTASAP SSAQVSGQPL SPSAQQAQQG LSPSHIQGSS
1021STQGQALQQQ QQQQQNSSVQ HTYLPSAWNS FRGYSSEIQM MTLPPGQFVI TDSGVATPVT
1081TGQVKAVTSG HYVLSESQSE LEEKQTSALS GGVQVEPPAH SDSLDPQTNS QQQTTQYIIT
1141TTTNGNGSSE VHITKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1156 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.